Beckman Coulter CEQ 8000, User Manual

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Fragment Analysis Procedures
284 CEQ™ 8000 Genetic Analysis System
Figure 156:Run LOH Analysis
Pairs of samples to be compared must have the same “root” names followed by
characters that allow the distinction between reference and test samples or loci. All
data to be analyzed will reside in a temporary file created by the CEQ System
Fragment Analysis application.
The macro scans the fragment results contained in the Study to determine the loci to
include in the LOH analysis. For each result in the Study, a list of alleles is then
generated at each locus. The peak heights of alleles identified in similarly named
samples are then compared and their status determined (LOH, AI, MI, Homozygous,
NoNorm Alleles).
You must have “Enable Macros” selected to run the LOH Analysis.
You will receive a prompt to enable this feature if it is not already
selected.
From the LOH.xls
START
sheet:
1. Set the LOH threshold (and the AI threshold if desired).
2. Specify the unique character combination used to identify Normal samples or loci
(i.e. Norm).
3. If Normal and “Tumor” samples are being run in the same well, check the
corresponding check-box. If Normal and “Tumor” samples are in different wells,
make sure the check-box is unchecked.
4. Click the
START
button.
5. As the analysis is being processed, do not use the keyboard or the mouse.
6. When the processing is complete, you may save the results.