Beckman Coulter CEQ 8000, User Manual

Page: 255 / 382

User’s Guide 241
Fragment Analysis Parameters - Advanced Tab
The Advanced tab is used to set additional fragment analysis parameters. From this
window you can assign dye mobility correction values to compensate for the varying
effects of different dyes on fragment mobility, select a standard mobility reference and
indicate whether dye spectra used should be estimated from sample data or from saved
system spectra.
Figure 138:Fragment Analysis Parameters window - Advanced tab
Mobility
1. Select the desired
Dye Mobility Calibration
from the drop-down list box.
• The Dye Mobility Calibration is used to select a specific calibration from
the options available in the list box and to adjust migration times or
mobility to compensate for the varying effects of different dyes on
fragment mobility.
• Select
PA ver.1
if analyzing samples made from primers labeled with the
phosphoramidite dyes D2-PA, D3-PA, D4-PA.
•
AE ver.2
provides an improved calibration for primers labeled with the
active ester dyes (D1, D2, D3 or D4) and should be selected to obtain the
most accurate size estimates for these primers.