Beckman Coulter CEQ 8000, User Manual

Page: 202 / 382

Sequence Analysis Procedures
188 CEQ™ 8000 Genetic Analysis System
Changing Heterozygote Display Color
The heterozygous bases will be bold and red, by default. You can change the color of
the heterozygotes in the base sequence pane by selecting
Tools | Heterozygotes
Display Color
. Select a new color and click
OK
. The color of the ambiguity codes will
be changed in the base sequence text.
You can search for heterozygotes by using the Heterozygote Backward
Search
and the Heterozygote Forward Search icons in the Standard
toolbar.
Pinning Results
Several analyses (single or batch) may be necessary before a given result is
satisfactory. The CEQ System Software provides the ability to either overwrite a
previous result, or produce additional result tabs.
1. To overwrite a previous result, click the pin in the left-hand corner of the result tab
to “unpin” the result. A reanalysis of that sample will overwrite the existing
result.
2. To produce a new results tab, click the pin to “pin” the result. Upon
reanalysis, a new tab containing the reanalyzed results will be created.
Performing a Batch Analysis
To analyze more than one sample simultaneously, perform the following steps.
1. Select
Analysis | Batch Analysis
from the menu.
2. In the
Batch Analysis Selection
dialog box:
a. Select a
Project
name from the drop-down menu at the bottom of the dialog
box.
b. Select the appropriate tab:
Sample Data
or
Sample Plate Results
.
c. Select
Enable
and enter a filtering start and stop date, if desired.
d. Highlight a sample name and click the right arrow to move the sample name to
the right-side listbox. Continue highlighting sample names and moving them to
the right-side listbox until you have selected all the samples for analysis.
e. Select
OK
.
6. In the
Working Analysis Parameters
dialog box, edit the parameters, if
necessary, then select
OK
.
Reanalyzing a Batch
To reanalyze a batch, perform the following steps.
1. After performing a batch analysis, select
Analysis | Reanalyze Batch
from
the menu.
2. Select a new parameter set or edit the currently selected set from the
Working
Analysis Parameters
dialog box to obtain different results.