Beckman Coulter CEQ 8000, User Manual

Page: 265 / 382

User’s Guide 251
If the
/- A
box is not checked , the software will identify one of the two
alleles as the true allele based on the
Use +A peak to call allele
check box. Other
peaks will be included in the fragment list as
unknown alleles
.
Use +A peak to call allele
Select this check box if the +A form is expected to be the higher peak and leave it
unchecked if the -A form is expected to be the higher peak.
If the
Use +A peak to call allele
box is checked , the software will identify the +A
peak as the true allele in the fragment list when the
/- A
box is also
checked . The other peak will then be designated as either -A accordingly.
If the
Use +A peak to call allele
box is not checked , the software will identify
the +A peak as the true allele in the fragment list when the
/- A
box is also
not checked . The other peak will be included in the fragment list as an
unknown
allele
.
If the
Detect Spurious Peaks
check box has been selected and the peak falls
below the maximum height designation for spurious peaks, the other peak will be
included in the fragment list as a
spurious peak
.
Size Adjustments
+A Detection Use +A Peak to Call Allel
e
Not Checked
Size InA
Checked
Use +A Peak to Call Allele
Checked
No Size Adjustment One nucleotide is subtracted from
the size of the allele in the allele
list.
Size InA
Not Checked
One nucleotide is added to the
size of the allele in the allele list. No Size Adjustment
Truth Table for SA Allele Identification
+A Detection
/- A
Checked
/- A
Not Checked
Use +A Peak to Call Allele
Checked
All fragments assumed to be +A.
The +A fragment is identified as the
allele in the allele table.
Fragments wA (if any) are
identified as -A peaks or spurious
peaks. All fragments assumed to be +A.
The +A fragment is identified as the
allele in the allele table.
Fragments wA (if any) are
identified as unknown alleles or
spurious peaks.