Beckman Coulter CEQ 8000, User Manual

Page: 347 / 382

Direct Control Procedures
User’s Guide 333
Injecting a Sample
1. Select
Direct Control | Inject
from the menu.
2. In the
Inject
dialog box:
a. Enter a value for the voltage in kV.
b. Enter a time duration in seconds.
c. Identify the position of the sample using the
Sample Set
spin controls and
then select
Inject
.
Performing a Separation
1. Select
Direct Control | Separate
from the menu.
2. In the
Separate
dialog box:
a. Enter a value for the voltage in kV.
b. Enter a time duration in minutes.
c. Identify the position of the buffer using the
Buffer Set
spin controls and then
select
Separate
.
Data will not be saved.
Replenishing the Capillaries with Gel
1. Select
Direct Control | Gel Capillary Fill
from the menu.
2. From the
Gel Capillary Fill
dialog box, select the
Buffer Plate
or
Wetting
Tray
radio button to identify the position where waste will be expelled from the
capillaries.
a. If
Buffer Plate
was selected, use the
Buffer Plate
spin controls to identify
the waste position in the buffer plate and then select
Fill
.
b. If
Wetting Tray
was selected, click the
Fill
button.
Purging the Manifold
1. Select
Direct Control | Manifold Purge
from the menu.
2. In the
Manifold Purge
dialog box:
a. Enter a volume in milliliters (mL) (0.4mL default).
b. Enter the number of cycles and then select
Purge
.
Performing an Optical Alignment
To align the lasers with the detection windows of the eight capillaries, perform the
following steps.
1. Select
Direct Control | Optical Alignment
from the Run menu.
2. To save the alignment data:
a. Select the
Autosave
check box.