Beckman Coulter CEQ 8000, User Manual

Page: 270 / 382

Fragment Analysis Procedures
256 CEQ™ 8000 Genetic Analysis System
The Filter Set
area is where the user-defined exclusion filters are selected and
applied to the data. Each column contains a menu of parameters to be applied as
exclusion filters. The system comes equipped with two pre-defined sets of exclusion
filters. Additional filter sets can be created, modified and saved.
The
Summary
area displays a table that identifies the number of analyses that have
been affected by the application of exclusion filters. As the result set is manipulated,
you can view the number of samples that have been excluded from your Study based
upon your filter set parameters.
The Filter Sets applied to the Result Set data (2nd Level Filtering) are
different from the Filter Sets applied to the Fragment List data (3rd
Level Filtering). To the Result Set you apply filters to an entire sample
which may contain numerous fragments. To the Fragment List you
apply filters to individual fragments that have been identified within
the various samples. See the “Fragment List” section of this manual or
Online Help for more information.
Applying Exclusion Filters to the Result Set Data
A Filter Set is a collection of any number of filters that are either pre-defined by the
system or user-defined. The purpose of the Filter Set is to exclude any results that do
not meet the criteria of the Study and to include results that do.
For example , suppose that your result set contains multiple samples collected under
two different sets of fragment analysis parameters. One of these parameter sets used
the SizeStandard-400 to determine the fragment sizes while the second parameter set
used the SizeStandard-600 to make the determination.
To exclude any data that were analyzed with the SizeStandard-600.
1. Open the drop-down list box in the
Name
column of the
Filter Set
area and
select the
SS
(Size Standard) command.
2. Open the drop-down list box in the
Operator
column of the
Filter Set
area and
select the “
=
” command.
3. Open the drop-down list box in the
Value(s)
column of the
Filter Set
area and
type in “
SizeStandard-600
”.
4. Click
Apply
at the top of the Filter Set area.
All samples in the fragment results table that used the SizeStandard-600 are
highlighted in teal (visible by selecting this parameter for display). This indicates that
they have been excluded from the Study. The
Excluded
column in the
Summary
area has increased by the number of samples using the SizeStandard-600.
There are many filters available and each must be applied using an operator and a
value. Any number of filters can be applied to a result set and the filter sets can be
saved for future use.
You can assign filter sets to filter out samples with excessive baseline noise,
excessively broad peaks, no size standard, out of calibration range, etc.