Beckman Coulter CEQ 8000, User Manual

Page: 149 / 382

Assigning Analysis Parameter Sets
User’s Guide 135
The first phase is to “coax” the sample into the capillaries. The second
phase is to perform the normal separation of the samples.
d. Click
OK
to exit the
Method
dialog box or continue with step 8 to make
additional changes to the method.
5. In the
Save As
dialog box:
a. Select a
Project Name
from the drop-down menu.
b. Enter a name in the name field.
c. Click
OK
.
Assigning Analysis Parameter Sets
Analysis parameter sets define the conditions specific to an experiment used in data
processing.
Sequence Analysis Parameter Sets define the start and end times for the
analysis of raw data, the threshold below which bases will be called as ‘N’s, and the
information necessary to detect the start of data to be analyzed: the delay between the
detected start of data and the start of data analysis, the signal to noise ratio, the
minimum duration, and the threshold above which data will be considered peaks.
Fragment Analysis Parameter Sets are used to process the fragment data to estimated
fragment sizes and to identify alleles. Parameter sets are created or edited in the
Fragment Analysis Parameters window. This window contains a number of tabs in
which you define the general parameter set information, set locus tag parameters,
select an analysis method to be used, assign quantitation values, and define several
other factors that will affect how your data will be analyzed.
For sequence analysis, select a Sequence Analysis Parameter Set. For fragment
analysis, select a Fragment Analysis Parameter Set.
Assigning a Parameter Set
To perform an analysis on a sample, select the cell/cells as described in “Sample
Selection’ on page 124 and select the
Perform Analysis
check-box, then click the
down-arrow on the Parameter Set (see Figure 79) to assign a Parameter Set to each
selected sample. Save the sample plate.
Figure 79: Analysis tab