Beckman Coulter CEQ 8000, User Manual

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User’s Guide 243
Only one set of system spectra is stored. This set of system spectra is automatically
used if one or more of the needed spectra cannot be estimated from the sample data.
• The
Use calculated dye spectra
option button is the default setting. This
selection uses the set of dye spectra obtained from the sample data.
• Select the
Use system dye spectra
option button and the
Dye
check boxes for
each dye trace you would like to be generated. With this option selected, no
attempt will be made to estimate the dye spectra from the sample being run, and
only the selected system dye spectra will be applied to the data.
• The system dye spectra can be established when the fragment test sample is run
during the installation process by viewing the Dye Matrices window for a sample
result in which spectra for all four dye labels were estimated. This set of dye
spectra can then be saved by the system by clicking on the
Save as System Dye
Spectra button.