Beckman Coulter CEQ 8000, User Manual

Page: 173 / 382

Analyzing Sequence Data
User’s Guide 159
The system will use the IUB ambiguity codes in Table 69 to represent heterozygous
bases.
The system will report the heterozygous bases in the Analyzed Data and in the Base
Sequence Data.
Alignment Template Tab
When the Alignment Template tab is clicked, the dialog box in Figure 96 is shown.
If
Automatic Alignment
is checked on the General tab, the options on this tab,
along with the information on the Alignment Parameters tab, will be used for the
alignment operation.
Table 69: IUB Ambiguity Codes
Code Definition Mnemonic
A Adenine A
C Cytosine C
G Guanine G
T Thymine T
R AG puRine
Y CT pYrimidine
K GT Keto
M AC aMino
S GC Strong 3 H bonds
W AT Weak 2 H bonds
B CGT Not A
D AGT Not C
H ACT Not G
V ACG Not T
N AGCT aNything