Beckman Coulter CEQ 8000, User Manual

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Sequence Analysis Procedures
150 CEQ™ 8000 Genetic Analysis System
Sequence Results: This tab displays the list of analyzed data that resides in the
selected project. On the right-hand side of the list box you may view the date and time
the sample was run as well as the raw data from which the sequence result was
derived. Sequence data consists of analyzed data and its base sequence text. When you
open sequence data you may also view the associated raw data, current, and voltage.
This option is available for sequence analysis only.
To filter the list, check the
Enable
check box and enter the
Start
and
End
dates for
the items you wish to view. Click
Refresh
to view the filtered results.
Sample Plate Results: This tab displays the list of sample plates run in the selected
project. Selecting a sample plate result displays the Sample Plate toolbar. Click on the
desired sample(s), then click on Open. The desired sample(s) are opened.
Sequence Analysis Parameters: Displays the Sequence Analysis Parameters saved in
the project. The Sequence Analysis Parameter Set determines the start and end times
for the analysis of raw data, the threshold above which data will be considered peaks,
the data start criteria, the heterozygote detection parameters and the alignment
template and parameters. You may also edit sequence analysis parameters under the
Analysis tab in the Sample Setup module.
Optical Scan Data: This tab lists the alignment optical scan data available in the
selected project. Optical scan data is the result of the two lasers scanning the
capillaries in order to determine the position of the capillary windows where the lasers
will focus when collecting data. If the scan is successful eight peaks are displayed for
each laser, denoting that all eight capillary windows were established.