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6-52
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Manual Calibration Worksheets
Section 6
Worksheet 1
Open Mode Calibration — New Factors
Instrument:
Date:
Operator:
1. In column 1, enter the calibrator assay values or the fresh whole blood reference means that were used in
the calibration process (refer to step 9 on page 6-45). Use the same WBC Reference Value for WOC and
NOC.
2. In column 2, enter the mean values calculated in the QC file.
3. In column 3, enter the calibration factors that existed prior to running the current calibration procedure.
4. For each parameter, divide the value in column 1 by the value in column 2 and multiply the result by the
value in column 3.
5. The value calculated in step 4 is the new calibration factor. Write this value in column 4.
6. Compare the new calibration factor in column 4 with the range shown in column 5. If the new factor falls
within the range, go to Worksheet 2. If the new factor falls outside the range, check all calculations. If
necessary, run the samples again into a new QC file and perform new calculations.
(Calculate All Factors To Three Decimal Places)
(1)
Assay Value
or
Ref Mean
/
(2)
Open Mode
Mean
x
(3)
Current
Open Mode
Cal Factor
=
(4)
New
Open Mode
Cal Factor
(5)
Range
WOC
/
x
=
0.700–1.300
NOC
/
x
=
0.700–1.300
RBC
/
x
=
0.800–1.200
HGB
/
x
=
0.700–1.300
MCV
/
x
=
0.700–1.300
PLT
/
x
=
0.700–1.300