6-42
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Enter Factor Method
Section 6
Confirming Open Mode Calibration
1. Press
[RUN]
followed by
[SPECIMEN TYPE]
. Select an empty QC file and press
[QC SPECIMEN]
.
2. Refer to Worksheet 7, Calibration Confirmation, in
Manual Calibration
Worksheets
provided at the end of this section.
NOTE:
Make copies of this worksheet as necessary.
3a. Obtain the calibrator used in the calibration process and run it three times into
the empty QC file. When the runs are completed, print the appropriate QC
file and transfer the mean values to the appropriate spaces on Worksheet 7.
3b. Obtain each of the five samples used in the calibration process and run each
sample once. When the five runs are complete, print the appropriate QC file
and transfer the mean values to the appropriate spaces on Worksheet 7.
4. Follow the instructions on the worksheet for comparing the mean value
against the assay values (calibrator), or reference mean (fresh whole blood).
5. If the difference between the mean of 3/5 runs and the assay value/reference
mean is within the tolerance limits, then calibration is confirmed for that
parameter. Proceed to calibrate the Closed Mode if not already calibrated.
NOTE:
Calibration of the Open Mode may also be confirmed by running
commercial controls.
6. If a parameter’s mean is outside the stated tolerance limits, repeat steps 3 and
4 above for that parameter. If the mean is still outside the stated tolerance
limits, call the Abbott Hematology Customer Support Center for assistance
(at 1-800-CELL-DYN in the U.S.).
