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CELL-DYN
®
3200 System Operator’s Manual
4-7
9140181H—October 2001
Section 4
Performance Characteristics and Specifications
Measurement Specifications — CELL-DYN 3200SL and CS
Measurement Channels
Laser optics for WBC and RBC/PLT
Hemoglobin Absorbance
Reticulocyte %
WBC (WOC)
Method
Laser light scatter
Light source
Vertically polarized 5–10 mW helium-neon laser
Wave length
632.8 nm
Dilution
1:50 of blood in WBC lyse
Data Collection
Four angles measured: 0°, 10°, 90°, and 90° depolarized. Data
collected in 256 channels for each angle of light scatter
WBC (NOC)
Method
Laser light scatter
Light source
Vertically polarized 5–10 mW helium-neon laser
Wave length
632.8 nm
Dilution
1:218 of blood in diluent/sheath and HGB Lyse
Data Collection
Four angles measured: 0°, 10°, 90°, and 90° depolarized. Data
collected in 256 channels for each angle of light scatter
RBCs and PLTs
Method
Laser light scatter
Light source
Vertically polarized 5–10 mW helium-neon laser
Wave length
632.8 nm
Dilution
1:1,675 of blood in diluent/sheath
Data Collection
Three angles measured: 0°, 10°, and 90°. Data collected in 256
channels for each angle of light scatter
HGB
Method
Modified methemoglobin
Light Source
LED, wavelength: 555 nm
Wavelength
555 nm
Dilution
1:218 of blood in diluent/sheath and HGB Lyse
Data Collection
Five absorbance readings for the blank, lowest and highest
readings eliminated. The three remaining absorbance readings
are averaged for the HGB reference. Five absorbance readings
for the sample, lowest and highest readings eliminated. The
three remaining absorbance readings are averaged for the
HGB sample.