CELL-DYN
®
3200 System Operator’s Manual
6-43
9140181H—October 2001
Section 6
Calibration Procedures
Calibration Procedure — Mode to Mode
Follow the procedure below for calibrating the instrument in the Closed Mode.
NOTE:
Use the same Calibrator or Fresh Whole Blood samples for calibrating
Open and Closed Modes.
Determining the Closed Mode Mean
1. If necessary, go to the
RUN
menu and press
[CHANGE SAMPLER]
to select the
Closed Mode.
2. In the
MAIN MENU
, press
[CALIBRATION]
to display the main
CALIBRATION
menu.
3. Obtain a printout of the current Closed Sampler Calibration Factors as
follows:
a. If necessary, press
[CLOSED SAMPLER]
to display the Closed Sampler
Calibration Factors.
b. Press
[PRINT]
to obtain a printout of these factors. Save this printout. It
will be used when completing Worksheet 6 at the end of this section.
4. Open an empty QC file as follows:
a. In the
MAIN MENU
, press
[RUN]
to display the
RUN
menu.
b. If necessary, press
[CHANGE SAMPLER]
to select Closed Mode.
c. Press
[SPECIMEN TYPE]
to display the
SPECIMEN TYPE
menu.
d. Use the arrow keys on the keyboard to move the cursor to an empty QC
file and type the desired name in the <File Name> field.
e. Press Enter to save the name. Use the Up Arrow key to move the cursor
back to the file name.
f. Press
[QC SPECIMEN]
to return to the
RUN
screen.
WARNING: Potential Biohazard.
Consider all specimens, calibrators,
and controls that contain human blood as potentially infectious. Wear
gloves, lab coats, and safety glasses and follow other biosafety procedures
as specified in the OSHA Bloodborne Pathogen Rule (29 CFR 1910.1030)
or other equivalent biosafety procedures.
5. Calibrating the CS model (Calibrator or Fresh Whole Blood):
a. Mix the specimen well. If calibrator material is used, follow the mixing
instructions found in the package insert. If fresh whole blood is used, mix
it well by inverting the tube at least ten times. Do not shake the specimen.
b. Place the well-mixed specimen in the Tube Retainer, close the door, and
press the Touch Plate.
c. If calibrator material is used, repeat this process until a minimum of 6
runs has been completed.
d. If fresh whole blood is used, repeat this process until a minimum of 10
runs has been completed.
e. Go to step 7.
