Beckman Coulter UniCel DxC 600, Инструкция по эксплуатации

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A13914AF
8-7
User Defined Reagents
Chemistry Parameters
8
Calculation Factor
A calculation factor should be entered if no calibrators are defined. If a calculation factor is not
defined, the system will not accept the default value of 0, and the user must type 1. For downgoing
reactions, the entry must be a negative number. Additional slope and offset adjustments may be
made at the
Reagent Status/Calibration Status
screen.
For calculating the appropriate factor for rate chemistries, a derivation of the Beer's Law formula
as applied to bichromatic chemistries can be used as follows:
Number of Calibrators
•
If no calibrators are used, type 0.
—
A linear math model allows no more than two calibrators.
—
A non-linear math model requires five or more calibrators.
• If two or more calibrators are used, span values are shown.
•
If five or more calibrators are used, recovery and sensitivity statistics are also shown.
where:
e
1
= The extinction coefficient of the chromophore at the primary wavelength.
e
2
= The extinction coefficient of the chromophore at the secondary wavelength.
Extinction coefficients for the chromophore MUST be obtained from the reagent manufacturer or
determined experimentally (refer to
Determination of Extinction Coefficients in this chapter).
T.V. = Total reaction volume (sample plus reagent, μL)
S.V. = Sample volume (μL)
0.5 = Cuvette pathlength (cm)
1000 = Units correction factor
Table 8.10 Allowable Entries for a Calculation Factor
-99999.000 to 99999.000, except 0
E014426L.EPS
×
0.5
(e
1
– e
2
)
1000
S. V .
T. V .
Table 8.11 Options for the Number of Calibrators
0, 1, 2, 3, 5, or 6