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MULTIPHOTON LASER SCANNING MICROSCOPY
LSM 510 META NLO
Using the LSM 510 NLO direct coupled system
Carl Zeiss
03/06
B 45-0021 e
9-15
Fig. 9-5
(A) Setup for checking the beam alignment; (B) Optimal shape of the NIR laser spot
Ideally this spot should be 5-7 mm in diameter and should be completely round and uniform. If the beam
appears to be clipped (flat on one side), small adjustments in the routing mirrors can improve the
alignment (see above). Shadows in the illuminated spot may indicate dust or debris on the routing optics
or in the scan head (check all visible elements for debris, then contact a service engineer if the problem
persists).
If proper alignment cannot be established using this protocol or if the image quality appears to be poor,
use the advanced alignment protocol to peak alignment.