MULTIPHOTON LASER SCANNING MICROSCOPY
LSM 510 META NLO
Using the LSM 510 NLO direct coupled system
Carl Zeiss
03/06
B 45-0021 e
9-23
Other molecules, such as salt crystals, can also focus light within tissues and intensify heating effects. This
can also lead to imaging artifacts, perhaps due to signal produced via second harmonic generation (SHG).
In Fig. 9-9 Calcium Carbonate crystals in the inner ear of the frog have produced blotchy artifacts.
A
B
Fig. 9-9
Multiphoton image (A) and stack of images (B) of the frog otolith. Cells have been
fluorescently stained by soaking the freshly prepared specimen in FM164 (arrow). Signal
produced by the artifact is shown inside the circle. Sample courtesy of Bill Roberts, University
of Oregon.
The artifacts completely obscure the three-dimensional reconstruction shown in Fig. 9-8/
B
.
This effect is not specific to Calcium Carbonate and has been seen in other samples, such as those with
crystals of salt from evaporated sea water (data not shown).
