OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Macro Menu
LSM 510 META
DuoScan
4-192
B 45-0021 e
03/06
4.8.4.7
Multiple Time Series Macro
The Multi Time Series program is designed to
provide flexible programming of automated time
dependent experiments.
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A pre-program time delay
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A pre-bleaching functionality
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An autofocus functionality
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A number of different, single time series
(called a block) either at the same position of
a sample or at different positions (if a
motorized stage is available)
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A time series of a tile scan
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A time series of a tiled Z stack
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A Z-Stack with triggered acquisition of each
single image in the stack
The basic programming unit is a single Time Series
Block in line, frame or Z-stack mode. In each block
the user can define a configuration for the data
acquisition (single or multi-track), the number of
images and the time interval/delay between
images.
The user can activate an optional autofocus
function before each block, pre-program a time
interval before each block (from the beginning of
the previous block to the beginning of the current
block), and/or execute a bleach track with
arbitrarily specified bleach ROI's, laser line(s) and
power.
The autofocus function can be executed using a
specified single-track configuration, or with the
configuration used for imaging (using the first
channel of the first track).
One can define the z-offset, the distance in the z direction from the position where the autofocus finds
the reference feature in focus (position of maximum intensity - e.g. position of the cover slip reflection in
the reflected light configuration) to the position, which is moved into focus plane when the image
acquisition begins (e.g. into the tissue).
The image acquisition is done with the configuration specified for the given block.
Autofocus search parameters: Z-offset as well as the Z-range (the distance in the z direction over which
the autofocus function searches for the plane of maximum intensity) can be set independently for
different stage locations (on the systems with the motorized stage).
Fig. 4-186
Multiple Time Series main dialog box
