LSM 510
ANNEX
LSM 510 META
NLO Non Linear Optics Laser for LSM 510 NLO and …
Carl Zeiss
03/06
B 45-0021 e
8-27
Operating Verdi Mira or Millenia Tsunami lasers
•
For other lasers: The laser itself is not switched on when clicking
On
, this has to be done directly at the
laser, but the software is informed that the laser is switched on and the laser safety shutter in the
AOM box can be opened.
•
Click the
Modify
button. The
Laser Modify Control
window is displayed (Fig. 8-12).
•
Enter the wavelength set manually on the laser in the
Edit
Laser Wavelength
input box.
•
The
Fine Tuning AO-Frequency
slider enables you to fine-tune the AO-frequency (Acousto Optical)
during the continuous scanning procedure. However This should only slightly influence the intensity of
the signal because the automatic presetting is calculated exactly for each wavelength.
•
Click
Store
to confirm the setting. The
Laser Modify Control
window is closed and the
Laser
Control
window is updated.
8.8.2 Configuration Control Window
Application of the NLO laser requires special main
dichroic beam splitters and the relevant emission
filters to be activated in the
Configuration
Control
window.
NLO-excited signals can be detected in every
channel by taking care on the blocking of the NIR
laser excitation light.
The following filters are especially designed for
detection of NLO-excited fluorescence signals:
−
HFT KP 680
main dichroic beam splitter reflecting NIR
excitation longer than 680 nm,
transmission for shorter wavelengths
−
HFT KP 700 / 488 (514, 543, 561) or
combinations of the KP 700 with more than
one additional laser line in the visible
range.
main dichroic beam splitter reflecting NIR
excitation longer than 700 nm,
transmission for shorter wavelengths and
reflecting 488 nm (or any other wavelength/s
indicated) excitation for simultaneous detection
of NIR and VIS excitation
−
KP 685
(short pass filter)
transmitting wavelengths shorter than 685 nm
−
BP 500-550 IR or other bandpass filters with indication of IR
special bandpass filters for NLO blocking also in the NIR range (extension IR),
can be used without additional BG 39
−
BG 39
highly efficient block filter for NIR, for combination with normal emission filters without IR extension
Fig. 8-13
Configuration Control window
