OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-90
B 45-0021 e
03/06
Acquisition of a Z Stack
Once you have set up your image as defined in the above section, you can collect a series of confocal
images through the different focal planes of your specimen.
•
Click on the
Start
button on the
Scan Control
window. The system will automatically start the
creation of a Z Stack. Be careful not to bump the air table or the microscope until Z sectioning is
completed. Each successive Z Slice can be viewed by changing to the
Gallery
mode. This button is
located on the right-hand side of the image.
A black bar will be shown under the image and will move from left to right, showing that the LSM 510 is
in the process. The laser will automatically stop scanning when the Z Stack is completed.
The entire stack of images can be saved using the
Save
or
Save As
buttons on the right-hand side of the
image.
Fig. 4-70
Image Display window of a Z Stack
