![background image](http://html1.mh-extra.com/html/zeiss/lsm-510/lsm-510_manual_3415651257.webp)
LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Macro Menu
Carl Zeiss
03/06
B 45-0021 e
4-195
Orientate panel
Find Focus b
utton
The objective chosen in the
Multiple Profiles Parameters...
window is
performed and a fast line scan over Z is acquired. The
Z range
of this line scan
is 0.5 mm, the single Z step 300 nm. The stage Z Position is set to the height of
the major maximum intensity position.
Find Gain
button
When clicking on the
Find Gain
button a
Find
procedure as described on page
4-72 is performed on the current
Z slice
.
Z-Settings panel
Z Range
[
μ
m]
This parameter defines the total
Z height
a
Multiple Profile
and/or a
Multiple
Stack
is performed.
Z range
values could be changed between 20 microns and
4 mm (if working distance of the objective will permit this, otherwise an error
message appears on the screen.
Because
in
Multiple Profile Scan
the single Z step could only be
varied from 100 … 600 nm and the maximum number of Z slices is
limited to 2000, the overall Z travel distance of the stage is also limited
to 200 … 1200
μ
m, respectively.
Sample Center
[%]
This button defines whether the actual Z position of the stage is rather placed
on top or on the button of the materials surface.
Multiple Profile panel
Profiles
The number of single profiles which has to be acquired, can be varied between
1 and 35. (Depending on the overlap total maximum size is restricted to less
than 32k).
Total Profile Length
The calculated total profile length is displayed
Multiple Stack panel
Stacks in X
The number of stacks in X dimension can be varied between 1 and 32.
Stacks in Y
The number of stacks in Y dimension can be varied between 1 and 32.
Multiple Profile button
If you click on the
Multiple Profile
button, the stage moves out of the center position of the sample and
is moved to the left-hand side to perform a first single profile. After that an interim image is performed
before and after the next step of the motorized XY stage. A cross correlation of the two profiles (Z) and
the two (not displayed) images (lateral) is performed to assure an accurate profile match online during
the experiment.
If you acquire the
Multiple Profile Scan
in
Range Indicator
palette the results of the
correlation could directly be observed as "blue boxes".