LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Macro Menu
Carl Zeiss
03/06
B 45-0021 e
4-193
4.8.4.8
StitchArt plus Macro
Purpose of the StitchArt plus Macro
The
StitchArt plus
Macro is useful for the acquisition of larger profiles and larger fields of view than a
single scan field could cover. It is applicable in reflection mode confocal imaging only.
With the StitchArt plus software profile lengths, which are required in standard tactile roughness
evaluation, could be performed (for instance: 12.5 mm profile length with 10x objective, 4.0 mm profile
length with 20x objective, 1.25 mm with 50x and 100x objective).
General requirements
−
The system and especially the lasers have to be in operation for at least one hour.
−
The motorized XY scanning stage has to be oriented as accurate as possible perpendicular to the
direction of the optical axes.
−
The parfocality adjustment of all objectives has to be performed and saved before running the
macro.
−
The pinhole has to be adjusted properly on a plane mirror or on the sample itself.
−
The calibration of the objective magnification in X and Y has to be performed (An image of an XY
calibration standard or another known length scale has to be taken and measured with the system
in X and Y direction. The ratio between the measured and the real length value has to be
calculated. The objective magnification has to be corrected with this factor and put in the objective
list as a new objective).
−
Pinhole diameter and amplifier offset have to be optimized by a prescan. They are automatically
taken from the
Channels
tab of the
Scan Control
window.
Epiplan Neofluar 20x/0.5
9 x 9 stacks
3.6 mm x 3.6 mm
Single scan field size
Fig. 4-187
StitchArt plus Macro
