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Philips CM12 (S)TEM 
Alignment & Setup 
Manual 
 

 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 

Summary of Contents for CM12

Page 1: ...Philips CM12 S TEM Alignment Setup Manual ...

Page 2: ...Erwin Sabio Philips CM12 Alignment Setup Manual Page 2 ...

Page 3: ...nt Column Alignment 9 Centering the Beam Alignment of Thermionic Gun Condenser Aperture Adjustment Condenser Astigmatism Correction Sample Loading and Unloading 11 Mounting the Specimen Inserting the Specimen Holder Sample Image Adjustment Optimizing Eucentric Height Adjusting Rotation Center Centering the Pivot Point Removing the Sample Obtaining the Image 14 Fine Tuning the Microscope Capturing ...

Page 4: ...older High vacuum tunnel where the specimen is inserted 4 Objective Aperture Assembly An area that houses the objective aperture control knobs and related circuitry 5 Field Limiting Aperture Assembly An area that houses the intermediate lens aperture control knobs and related circuitry 6 Binoculars Eyepieces used to view the projected image up close 7 Viewing Window Glass windows another on the le...

Page 5: ... Figure 3 The main control panel on the right hand side of the microscope 9 Intensity Knob 16 Emission Meter 10 Fine Adjustment Knob 17 High Tension Button 11 Screen 18 Filament Control Knob 12 Screen Control Buttons 19 Ready Button 13 Power Switch 20 Focus Focus Step Knob 14 Magnification Knob 21 Multifunction Control Knob 15 Beam Shift Knobs 9 10 11 12 16 17 4 18 15 19 20 21 13 14 ...

Page 6: ...en Holder Figure 4 The CM12 Specimen Holder 22 Black Cap 24 Specimen Cavity Clamp 23 O ring The Side Entry Specimen Chamber Figure 5 The Side Entry Chamber 25 Tilt Knob 27 Z Adjustment Knob 26 Indicator Light 28 Tilt Lock Lever Startup Procedure 22 23 24 26 25 27 28 ...

Page 7: ... be less than 35 5 Before powering the gun go to MODE TEM PARAMETER from the main screen to check the current and voltage setting that will be applied to the gun Ideally the current should be around 3 and the voltage at 100keV and should be kept at these levels Figure 7 The needle will kick twice to the right after the High Tension button is turned on 6 Finally push the HIGH TENSION 17 button to t...

Page 8: ...green beam seen at the viewing screen represents visually the beam of electrons 4 As the filament saturation is being adjusted check the green beam on the large screen inside the viewing chamber 7 The brightness of this beam indicates the relative intensity of the actual electron beam Turn the filament knob to reach just enough brightness of the beam Turning the knob above the FIL LIMIT will not i...

Page 9: ...rt by centering the cross over beam as described earlier Figure 9 The shadow of the filament becomes visible at low saturation 2 Undersaturate the gun by going to MODE CONFIGURATION and turning the Filament Knob 18 counter colckwise until the shadow of the filament becomes visible in the beam as in Figure 9 Figure 10 The STIG DF and ALGN buttons activate the multi function knobs 3 Press the ALGN b...

Page 10: ...either direction past the cross over Condenser Astigmatism Correction Figure 12 Schematic of the symptom of condenser astigmatism 1 Asymmetric magnetic fields around the condenser lens results in an unbalanced focus of the beam This phenomenon is called astigmatism When this happens the beam spreads as asymmetric ellipses on either direction past the cross over when the intensity knob is turned St...

Page 11: ...tly lower the clamp in place using the pin tool to ensure the sample is locked in place Inserting the Specimen Holder 1 Before inserting the specimen holder into the side entry chamber 3 also called goniometer check to make sure the tilt 25 is at 0 degrees and the lever 28 is in lock position 2 Grab the end of the specimen holder nearest the black cap 22 and ensure that the slit on the cap is at t...

Page 12: ...rection Otherwise turn the other way 4 Continue alternating between turning the z knob and tilting the device until the movement of the reference point reaches a minimum At this point the optimal z height has been reached 5 Return the tilt to 0 degree then put the lever in lock position Sample Image Adjustment Adjusting Rotation Center 1 As in z height adjustment begin by choosing a central featur...

Page 13: ... beam splits into two then the pivot point needs to be aligned Use the multifunction knobs to coincide the beams 3 Next select PIVOT PT Y and again coincide the beams if necessary using the multifunction knobs 4 Press the ALGN button again to exit the align mode Removing the Sample 1 Ensure once again that the tilt of the sample is at 0 and the level is in lock position 2 Pull the black cap of the...

Page 14: ...ad the beam to illuminate a wide area and take out the viewing screen out of the way of the camera by pulling a lever on the left side of the viewing chamber 2 Switch to the computer and access the iTEM imaging software Click on the ACQUIRE button to see a live image of the sample on the computer screen 3 If ready click on SNAPSHOT to capture a still image of the sample 4 Click on STOP ACQUISITION...

Page 15: ...ob counter clockwise until the filament level goes back to zero Note There is no wait time when decreasing the filament saturation 4 Turn off the HIGH TENSION switch and turn off the control panel If another user will use the microscope soon after just press VACUUM from the main menu to see the vacuum status then leave it at that 5 Cover the viewing windows with their lids 6 Logoff from the comput...

Page 16: ...S 2007 January 19 230L first report Email attachment 6 Author s photo 7 Ibid 8 Ibid 9 Williams David B and Carter C Barry 1996 Transmission Electron Microscopy Volume I p 74 New York Plenum Press 10 Author s photo 11 Williams p 137 12 Ibid 13 Gilder Grids Retrieved 2007 January 17 from http www gildergrids co uk 14 Untitled Retrieved 2007 January 22 from http www kwc edu academic biology TEMpics t...

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