Zeiss Axioskop 2 mot plus User Manual Download

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Operating Manual

Axioskop

plus

Axioskop

mot

plus

Routine Microscope

Summary of Contents for Axioskop 2 mot plus

Page 1: ...Operating Manual Axioskop 2 plus Axioskop 2 mot plus Routine Microscope...

Page 2: ...ot covered by an update service Unless expressly authorized forwarding and duplication of this document and the software package and the utilization and communication of its contents are not permitted...

Page 3: ...view of Axioskop 2 plus 0 13 Overall view of Axioskop 2 mot plus 0 14 CHAPTER 1 INSTRUMENT DESCRIPTION 1 2 1 1 Name and intended application 1 2 1 2 Instrument description and main features 1 2 1 3 Ax...

Page 4: ...2 2 1 Changing the condenser 2 16 2 2 2 Changing the phase stop for phase contrast or darkfield in the universal condenser 2 17 2 2 3 Changing the DIC prism in the universal condenser 2 18 2 2 4 Attac...

Page 5: ...ht darkfield 3 27 3 3 3 Setting of transmitted light phase contrast 3 29 3 3 4 Setting of transmitted light polarization 3 31 3 3 5 Setting of transmitted light differential interference contrast DIC...

Page 6: ...ENANCE TROUBLESHOOTING AND SERVICE 4 2 4 1 Instrument care 4 2 4 2 Instrument maintenance 4 3 4 2 1 Performing checks 4 3 4 2 2 Changing the fuses 4 3 4 3 Troubleshooting 4 4 4 4 Spares consumables an...

Page 7: ...ch a way that the specimen is not touched by the condenser 5 Close luminous field diaphragm until it is visible in the field of view even if not in focus Fig 0 1 A 6 Lower universal condenser 0 9 unti...

Page 8: ...s of the directive 98 79 EC and are marked with the marking This operation manual includes information and warnings which must be observed by the user The following warning and information symbols are...

Page 9: ...advertent operation Please contact a Zeiss service agency or Carl Zeiss Microscopy Service for repairing the instrument The power unit which is integrated in the stand of the microscopes permits the u...

Page 10: ...g special attenuation filters When hot gas discharge lamps are under high internal pressure and may therefore only be changed when they have cooled down and protective gloves and goggles must be used...

Page 11: ...d in its performance or destroyed when handled improperly Be sure to read the safety notes provided with Immersol 518 N immersion oil Immersol 518 N immersion oil irritates the skin Avoid any contact...

Page 12: ...antee is provided for defects caused by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any othe...

Page 13: ...p 2 plus Carl Zeiss B 40 075 e 10 03 0 13 Overall view of the Axioskop 2 plus 1 Eyepieces 2 Binocular tube 3 Stand 4 Incident light illuminator 5 Transmitted light illuminator 6 Manual universal conde...

Page 14: ...s Axioskop 2 mot plus 0 14 B 40 075 e 10 03 Overall view of the Axioskop 2 mot plus 1 Eyepieces 2 Binocular ergotube 3 Stand 4 Incident light illuminator 5 FL excitation filter wheel 6 Transmitted lig...

Page 15: ...ription and main features 1 2 1 3 Axioskop 2 plus manual microscope configurations and modules 1 4 1 4 Axioskop 2 mot plus microscope configurations and modules 1 8 1 5 Objectives 1 12 1 6 Eyepieces 1...

Page 16: ...rn demands on design operating convenience and function with technical performance Thanks to the modular design existing microscope modules of the Axioskop 2 plus such as tubes intermediate tubes and...

Page 17: ...n Plan Neofluar Plan Apochromat Binocular tubes and phototubes with fixed and variable viewing angle 10x focusing eyepieces with field number 23 suitable for spectacle wearers High performance epi flu...

Page 18: ...ioskop 2 plus microscope configurations Axioskop 2 mot plus 1 4 B 40 075 e 10 03 1 3 Axioskop 2 plus manual microscope configurations and modules Fig 1 1 Axioskop 2 plus manual microscope configuratio...

Page 19: ...Eyepiece 3 Binocular tube 30 23 4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen lamp 7 FL filter slider 8 Objective 9 DIC slider 10 Compensator 11 Dust cover 12 DIC prism 13 Ach...

Page 20: ...40x Plan Apochromat 63x and Plan Neofluar 100x Modules The Axioskop 2 plus routine microscope consists of the following modules Axioskop 2 plus microscope stand with 5x reflector turret harmonic drive...

Page 21: ...ting and fixed coaxial drive 130 mm 453505 9901 000 453502 9905 000 453523 0000 000 Specimen holder with spring clip R 453533 0000 000 Technique modules FL P C reflector module e g with filter sets fo...

Page 22: ...oskop 2 plus Carl Zeiss Axioskop 2 mot plus microscope configurations Axioskop 2 mot plus 1 8 B 40 075 e 10 03 1 4 Axioskop 2 mot plus microscope configurations and modules Fig 1 2 Axioskop 2 mot plus...

Page 23: ...6 25 23 4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen illuminator 7 FL filter slider 8 Objective 9 DIC slider 10 Compensator 11 Dust cover 12 DIC prism 13 Achromatic aplanati...

Page 24: ...C 100 W power unit MOT stabilized transmitted light equipment stage and condenser carrier FL incident light and HBO XBO adjusting aid 452311 0000 000 Eyepieces W PL 10x 23 Br foc PL 10x 23 Br foc 0000...

Page 25: ...or epi fluorescence filter set 01 UV excitation H 365 filter set 09 blue excitation 450 490 filter set 15 green excitation H 546 DIC FL analyzer Analyzer module D Optovar module 1 25x Optovar module 1...

Page 26: ...ite Magnification factor 1 25x 2 5x 4x 5x 6 3x 10x 16x 20x 25x 32x 40x 50x 63x 100x 150x The objective magnification multiplied with the eyepiece magnification minimum 10x results in the visual overal...

Page 27: ...n Transmitted light brightfield Achroplan 4x 0 10 11 1 440020 0000 000 Achroplan 10x 0 25 4 8 440030 0000 000 Achroplan 20x 0 45 2 07 0 17 440040 0000 000 Achroplan 40x 0 65 0 59 0 17 440050 0000 000...

Page 28: ...t image flatness up to the edge of the field of view PL 10x 23 Br foc eyepieces ensure excellent optical correction particularly in combination with the ergotubes If required eyecups for the eyepieces...

Page 29: ...nce 0 001 mm 474066 9901 000 Net micrometer 12 5x12 5 5 10 d 26 mm area 12 5x12 5 mm divided in fields of 10x10 474068 0000 000 Photo reticle MC 2 5x d 26 mm for 35 mm photography with an additional m...

Page 30: ...x 18 4 kg Axioskop 2 mot plus approx 22 4 kg Ambient conditions Transport in packaging Permissible ambient temperature 40 to 70 C Storage Permissible ambient temperature 10 to 40 C Permissible humidit...

Page 31: ...HBO 50 transformer mbq52ac z Operating environment clean room Protection class I Protection type IP 20 Line voltage can be changed between 100 110 120 127 VAC and 230 240 VAC Line frequency can be ch...

Page 32: ...7 positions for brightfield darkfield for phase contrast 1 2 3 for DIC I II III changeable Objective change manually via 6 position nosepiece H DIC W 0 8 Change of technique modules manually via 5 po...

Page 33: ...Changing the condenser 2 15 2 2 2 Changing the phase stop for phase contrast or darkfield in the universal condenser if required 2 16 2 2 3 Changing the DIC prism in the universal condenser 2 17 2 2 4...

Page 34: ...rear 2 14 Fig 2 22 Changing the condenser 2 15 Fig 2 23 Attachment of condenser MOT 2 16 Fig 2 24 Changing the phase stop 2 16 Fig 2 25 Changing the DIC prism 2 17 Fig 2 26 Attachment of filter mount...

Page 35: ...tube objectives eyepieces condenser 12 V 100 W illuminator microscope mat and various small parts e g DIC slider spare lamp dust cover tools bag The following components are factory attached to the m...

Page 36: ...4 Turn the binocular tube in the required observation position and tighten the hexagonal screw using the ball headed screwdriver 2 1 3 Insertion of eyepieces and centering telescope Remove both dust...

Page 37: ...eyepiece reticle If eyepiece reticles are inserted into the unscrewed mount by the customer attention must be paid to the labelling being visible the right way up after insertion Compensation of amet...

Page 38: ...gotubes Furthermore the binocular component of the ergotubes can be swivelled by 180 2 1 6 Screw in of objectives Move mechanical stage with carrier to the lower stop position Remove dust caps 2 6 2 f...

Page 39: ...er 2 7 2 to the lowest position via drive for height adjustment 2 7 5 Insert condenser 2 7 1 between condenser carrier 2 7 2 and stage carrier 2 7 4 Orient the locking screw on the underside of the co...

Page 40: ...automatically to the available line voltage within this range The HBO 103 illuminator for epi fluorescence is supplied via a separate transformer The HBO 100 W transformer must be connected to the lin...

Page 41: ...halogen illuminator a Coarse adjustment Loosen clamping screw 2 11 2 and remove the halogen illuminator from the microscope stand Switch on microscope as described under 0 Direct the light beam agains...

Page 42: ...nator 1 Attach HBO 103 W 2 mercury vapor short arc lamp The HBO 103 illuminator and the HBO 103 W 2 mercury vapor short arc lamp are supplied in separate packages for safety reasons Therefore insertio...

Page 43: ...oarse adjustment Remove HBO 103 illuminator from the microscope stand by loosening clamping screw 2 13 2 Switch on HBO 103 on HBO 100 W transformer 2 10 1 and allow it to heat to operating temperature...

Page 44: ...d as described in section 2 1 11 2 Pull adjusting aid 2 15 2 out of the incident light tube 2 15 1 The brighter focal spot of the HBO 103 W 2 lamp and its slightly darker reflector image become visibl...

Page 45: ...stand as described in section 2 1 11 2 Remove one objective from the nosepiece and switch empty opening in function position Place a white sheet of paper on the mechanical stage Fully open the precen...

Page 46: ...21 3 and combined CAN SUB BUS 2 21 1 connectors 2 1 13 Attachment of round cable holder The round cable holder supplied with the mechanical stages is suitable to hold and attach electric cables e g c...

Page 47: ...e left of the stand of connecting cable and pull off the connecting cable Unscrew both centering screws 2 22 3 until condenser can be easily taken out of its mount Lift available condenser from the or...

Page 48: ...oosen the cover on the condenser underside 2 24 2 loosen both grub screws and remove the cover Position turret disk with phase stop to be changed in the exchange opening and hold it on the knurled rin...

Page 49: ...ve DIC prism 2 25 2 from the tool and screw on the new required DIC prism Installation of the DIC prism is made in reverse order Make sure that the DIC prism is oriented correctly groove 2 25 5 must e...

Page 50: ...op bolt 2 27 2 with the adjusting lever 2 27 1 as far as the stop in the rear threaded hole of the condenser carrier 2 27 3 2 2 6 Attachment of SENARMONT DIC polarizer Hold polarizer 2 28 6 parallel t...

Page 51: ...t go the spring lever and pull the protection cap off Press spring lever briefly downwards again to center the lamp Attach lamp housing again and tighten clamping screw 2 29 5 2 2 8 Changing the HBO 1...

Page 52: ...clamping screw 2 31 2 and remove HBO 103 illuminator 2 31 3 from the incident light tube 2 31 1 of the microscope stand Use focusing knob 2 32 3 to bring collector to the position at the very front i...

Page 53: ...apply force to the lamp The holder in the heat sink is marked with H for HBO or X for XBO Clamp the fixing screw on the heat sink Insert the new lamp 2 33 1 with heat sink with the spring clip 2 33 4...

Page 54: ...rier 2 34 6 in such a way that the threaded holes 2 34 2 on the underside of the stage are positioned above the openings of the stage carrier 2 34 3 Insert four fixation screws 2 34 5 through the stag...

Page 55: ...centering should be performed as follows Loosen stage clamping screw 2 36 4 and screwing cap of the stage carrier 2 36 1 Rotate the stage to determine the maximum specimen deflection 2 36 5 tip of the...

Page 56: ...e additional sleeves back on the drive knobs in reverse order and tighten both the clamping screws 5 Setting the smoothness torque of the two drive knobs of the ergo drive The ergo drive is set to a m...

Page 57: ...38 2 from the mechanical stage 2 38 4 in an upward direction Attach required specimen holder 2 38 5 to the mechanical stage and tighten two countersunk screws 2 2 10 Changing the binocular tube Use S...

Page 58: ...ovetail of the intermediate tube with height adjustment 2 40 5 Insert dovetail of intermediate tube in the stand opening 2 40 8 and tighten clamping screw 2 40 7 only slightly Align intermediate tube...

Page 59: ...n upward direction Insert dovetail of the adapter for the Axioskop 2 plus 2 41 3 in the stand opening 2 41 6 on the underside and slightly tighten clamping screw 2 41 5 Insert fixation screw 2 41 2 in...

Page 60: ...et Then press the module at the top until it also snaps securely into the top spring clamps of the reflector turret Aligning the respective module when installing it in the reflector turret FL P C ref...

Page 61: ...e The barrier filter is inserted at 2 43 2 the excitation filter at 2 43 4 and both are secured using retainer ring 2 43 1 2 2 15 Changing the beam splitter in the FL P C reflector module Attachment o...

Page 62: ...module half Make sure that the side latch of the spring frame is in the appropriate recess in the bottom module half The reflective side of the beam splitter is recognizable by carefully placing a wo...

Page 63: ...lamping screw 2 46 12 to prevent the coobservation equipment from shifting Unscrew the tube lenses from the two binocular tubes using the cover of one of the storage cases and put them in the cases Re...

Page 64: ...equipment for 2 users 1 Binocular ergotube 2 Binocular tube 3 Dust cap 4 Rear dovetail 5 Socket for power supply 6 Heat protection shield 7 Fixation screw 8 Clamping screw 9 Support point stand 10 Pow...

Page 65: ...en the captive coupling nuts Attach and clamp two binocular tubes for the multi observation equipment 2 47 6 on each of the connecting pieces L 2 47 5 and R 2 47 13 When mechanically aligning the conn...

Page 66: ...0 075 e 10 03 1 Clamping screw 6 Coobservation tube 11 End connector R 2 Clamping screw 7 End connector L 12 Setting screw 3 Dovetail 8 Socket for power supply 13 Connecting piece R 4 Binocular tube 9...

Page 67: ...tion 2 48 2 on the stand and tighten clamping screw 2 48 10 only slightly at first Centering is performed using the dovetail Insert second clamping screw with washer 2 48 3 in the drilled hole provide...

Page 68: ...START UP Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus 2 36 B 40 075 e 10 03...

Page 69: ...ght darkfield 3 27 3 3 3 Setting of transmitted light phase contrast 3 29 3 3 4 Setting of transmitted light polarization 3 31 3 3 5 Setting of transmitted light differential interference contrast DIC...

Page 70: ...the height stop on the condenser carrier 3 26 Fig 3 19 Centering of darkfield stop on the universal condenser 3 28 Fig 3 20 Centering of phase stop on universal condenser 3 30 Fig 3 21 Centering of ph...

Page 71: ...a manual version and in a version with motorized coded components In the Operation chapter the manual setting and operation functions are explained first and the motorized coded functions are explaine...

Page 72: ...Operation and function controls Axioskop 2 mot plus 3 4 B 40 075 e 02 01 3 1 1 Operation and function controls of the Axioskop 2 plus Fig 3 1 Operation and function controls of the Axioskop 2 plus 1 0...

Page 73: ...tensity 12 Color temperature key 3200 K 13 Focusing drive coarse adjustment on both sides 14 Focusing drive fine adjustment on both sides 15 Lever to swing in out the diffusion disk 16 Drive for movem...

Page 74: ...inous field diaphragm diameter see section 3 3 6 Pushrod pushed in fully open Pushrod pulled out closed Compartment for filter slider For 3 position or 6 position FL filter slider with 18 mm filter di...

Page 75: ...ted diffusion disk 3 1 15 is used to achieve homogeneous illumination though at a slight loss of light Lever in front position diffusion disk swung out Lever in rear position diffusion disk swung in D...

Page 76: ...rference contrast DIC I II III aperture diaphragm iris stop The front lens is folded in out using lever 3 2 2 Turning the turret wheel 3 2 4 swings the brightfield insert and the contrast stops in the...

Page 77: ...nt light and optovar module and analyzer module for transmitted light Fast change of technique modules by turning the knurled ring of the reflector turret Luminous field diaphragm Wheel 3 5 2 for the...

Page 78: ...arl Zeiss Operation and function controls Axioskop 2 mot plus 3 10 B 40 075 e 02 01 3 1 2 Overview of operation and function controls of the Axioskop 2 mot plus Fig 3 6 Operation and function controls...

Page 79: ...e clamping ring Close the filter housing and tighten the knurled screw LED lamp voltage array Indicates the present operating voltage of the halogen illuminator in 10 steps 3 12 V Indicates the speed...

Page 80: ...3 7 1 aperture diaphragm is opened until stop as long as the key remains pressed Key close aperture diaphragm 3 7 2 aperture diaphragm is closed until stop as long as the key remains pressed Key front...

Page 81: ...key is activated the stage is moved downwards out of the focus position by approx 6 mm The current focus position is stored The specimen can be changed When the stage is in the low position the motori...

Page 82: ...is activated the transmitted light is switched on or off Transmitted light is always available when the microscope is switched on Set key Stores illumination and condenser settings and is used for pr...

Page 83: ...pieces or to the attached camera via a pushrod Pushrod 3 10 2 pushed in 100 of the light for the eyepieces Pushrod 3 10 2 pulled out 100 of the light for the camera To set the most convenient viewing...

Page 84: ...4400 when used individually or in a combination When several filters are combined the transmission value is calculated as the product of the individual transmission values in percent Filter slider The...

Page 85: ...ed continuously from 60 to 110 mm This intermediate tube can only be used in combination with the binocular ergotube 6 25 23 or the binocular ergo phototube 6 25 23 100 100 Loosen clamping lever 3 13...

Page 86: ...check of all the connected components During the system check the pilot lamp on the stand lights up in orange After approx 3 seconds the microscope is ready for operation and is in the so called auto...

Page 87: ...le beep is heard Settings Light intensity depending on objective nosepiece and technique module reflector turret Condenser turret position depending on objective nosepiece and technique module reflect...

Page 88: ...enser turret Switching of condenser turret Folding in out of front lens Transmitted light on off Opening closing of aperture diaphragm transmitted light The values for illumination intensity and the c...

Page 89: ...mming mode When the parameters for stage focusing are set individually the focus positions of the various objectives parfocality and the focusing speeds are matched to the appropriate objective magnif...

Page 90: ...10 steps via the two keys for fast stage lowering lifting 3 14 2 Key higher speed Key lower speed The focusing speed can be matched to the individual requirements by switching between coarse and fine...

Page 91: ...g mode is confirmed by a beep when the Set key is released The pilot lamp lights up in green again If several reflector positions are to be used e g different reflector modules for incident light or O...

Page 92: ...to K HLER on the manual Axioskop 2 plus 2 Transmitted light brightfield equipment of the manual Axioskop 2 plus Each manual Axioskop 2 plus configuration permits performance of the transmitted light b...

Page 93: ...into the tube with your naked eye Use sliding knob 3 17 5 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupils 3 17 E In most applications this aperture diaphr...

Page 94: ...s the matching of focus positions and focus speeds of the used objectives has been finished The instrument is switched on and is in the automatic mode Illumination intensity condenser settings positio...

Page 95: ...eld settings on the manual Axioskop 2 plus and Axioskop 2 mot plus Setting of K HLER illumination is identical to transmitted light brightfield except that the highest aperture objective must be used...

Page 96: ...and the luminous field diaphragm image will be almost perfectly in focus Finally match the diameter of the luminous field diaphragm to the size of the field of view Darkfield microscopy requires spec...

Page 97: ...s optical channel and are influenced by the in phase refractive index and the thickness differences in the specimen In the intermediate image plane the differently influenced partial beams interfere a...

Page 98: ...f congruence is not perfect 3 21 A the two SW 1 5 screwdrivers 3 20 1 and 4 must be used on the two centering screws 3 20 2 and 3 to recenter the bright phase stop until complete congruence with the d...

Page 99: ...uration Objectives suitable for polarization Rotary mechanical stage 75x50 240 R SENARMONT DIC polarizer or polarizer D rotary or fixed unless a polarizer is integrated in the condenser Analyzer modul...

Page 100: ...ary or fixed unless a condenser with integrated polarizer is used Analyzer module D in reflector turret Adjusting specimen for polarization microscopy 453679 0000 000 3 Setting of Axioskop 2 plus and...

Page 101: ...e largest vibration direction n defined to be oriented in NE SW direction The specimen changes its color when the compensator is pushed in The type of the color change depends on the orientation of th...

Page 102: ...imen to be examined is parallel to the largest vibration direction of the compensator i e in NE SW direction the path difference of the specimen e g grayish white 150 nm and the path difference of the...

Page 103: ...of Axioskop 2 plus and Axioskop 2 mot plus Set the microscope as for transmitted light brightfield according to K HLER see section 3 3 1 3 Center the rotary stage 3 25 3 see section 2 2 9 2 and then...

Page 104: ...skop 2 mot plus configuration Objectives offered with DIC equipment e g Plan Neofluar Nosepiece with compartment for DIC slider DIC slider suitable for the objectives used Condenser with turret disk w...

Page 105: ...ning the polarizer via the lever in the lower part of the SENARMONT polarizer 3 26 7c The 4 plate above it must always be switched on for SENARMONT contrasting to function If the DIC slider was moved...

Page 106: ...6 0 7 0 8 0 90 10 0 1 Objective in nosepiece 7a DIC prism with polarization foil 2 Reflector turret in the condenser or 3 Analyzer module 7b D polarizer 4 DIC slider 7c SENARMONT polarizer 5 Knurled s...

Page 107: ...UV excitation FL P C reflector module and shutter plate in the reflector turret HBO 103 or HBO 50 mercury vapor short arc lamp for incident light illumination HAL 100 halogen illuminator for transmit...

Page 108: ...d Insert eyepiece in tube again and close luminous field diaphragm 3 27 5 until it is visible in the field of view Use the two centering screws 3 27 6 to center the luminous field diaphragm in relatio...

Page 109: ...of a SLR camera e g CONTAX 167 MT Screw T2 adapter 416010 0000 000 3 28 3 suitable for the used camera on the 2 5x connector for T2 456005 0000 000 3 28 4 Attach camera 416181 0000 000 3 28 2 and if...

Page 110: ...cameras T2 Adapter for SLR cameras Cat No T2 Adapter for CONTAX CONTAX bayonet 416010 0000 000 T2 Adapter for OLYMPUS OM OM bayonet 416002 0000 000 T2 Adapter for MINOLTA SR bayonet 416003 0000 000 T2...

Page 111: ...h resolution CCD cameras to the phototube of the Axioskop 2 plus Axioskop 2 mot plus Tube Adapters Cameras 456105 456105 0000 000 60 C 2 3 1 0 456107 000000 1069 414 60 C 2 3 0 63 456106 456106 0000 0...

Page 112: ...40 456140 0000 000 Adapter 60 44 Microscopes with 60 mm interface and all video adapters for 44 mm interface Connects video adapters for 44 mm interface to microscopes with 60 mm interface 3 4 3 Attac...

Page 113: ...6 25 23 3 29 1 or binocular ergo phototube 6 25 23 to the front tube mount of the microscope Attach binocular tube 3 29 2 or phototube 30 23 to the rear tube mount of the microscope The binocular erg...

Page 114: ...pment Axioskop 2 mot plus 3 46 B 40 075 e 02 01 1 Binocular ergotube 2 Binocular tube 3 Basic body 4 Power unit 5 Knob for brightness control 6 Lever for light pointer Fig 3 29 Active coobservation eq...

Page 115: ...nector L 3 30 8 left Binocular tubes 30 23 for further viewing stations 3 30 6 and 7 452927 0000 000 We would recommend you to have the multi observation equipment assembled by Carl Zeiss service staf...

Page 116: ...B 40 075 e 02 01 1 Knob for brightness control 7 Coobservation tube 2 Basic body 8 End connector L 3 Lever for light pointer 9 Power unit 4 Binocular tube 10 End connector R 5 Connecting piece L 11 C...

Page 117: ...e microscope This enables the user to copy microscope specimens by drawing Furthermore transparent references e g grain size plates reference scales for photomicrography or other self made patterns wi...

Page 118: ...is scale value is exactly that distance in the specimen which complies to one interval of the used crossline micrometer For calibration align the scales of the stage micrometer and the crossline micro...

Page 119: ...nts using the microscope are always possible to be performed if both the lower and upper side of the specimen can be focused This should preferably be performed using a precision Harmonic Drive focusi...

Page 120: ...ry objective nM 1 0 The indicated intervals of the mechanical fine drive are 15 0 and 24 5 which means that d 9 5 m with an interval of 1 m The refractive index of the specimen area nP has been assume...

Page 121: ...INTENANCE TROUBLESHOOTING AND SERVICE Contents 4 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE 4 2 4 1 Instrument care 4 2 4 2 Instrument maintenance 4 3 4 2 1 Performing checks 4 3 4 2 2 Changing the...

Page 122: ...Optics cleaning solution consisting of 15 isopropanol and 85 medical alcohol benzoline clean glass surface using a slightly moistened wad of cotton by moving in circles starting in the middle Remove...

Page 123: ...ke sure that the maximum operation time of the halogen and mercury vapor short arc lamps is not exceeded 4 2 2 Changing the fuses Always pull the power plug before changing the fuses The fuse compartm...

Page 124: ...the phototube in the correct position end position see page 3 15 visible Nosepiece with objective not in click stop position Switch nosepiece with objective in click stop position Condenser not set c...

Page 125: ...Use of no or unspecified immersion oil with immersion objectives Use immersion oil 518 N from Carl Zeiss see p 4 8 Air bubbles in the immersion oil Apply new oil to remove the bubbles Immersion oil at...

Page 126: ...e ametropia s p 2 5 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set interpupillary distance correctly see p 2 6 Focusing eyepieces are not set correctly...

Page 127: ...top nosepiece in the required position The 12 V 100 W halogen lamp of the Axioskop 2 mot plus does not function although the on off switch is in on position Reflector turret is in epi fluorescence pos...

Page 128: ...444801 0000 000 recommended for low brightness techniques to suppress reflected light Dust cover for nosepiece Dust cover for eyepiece tube 462981 0000 000 000000 0168 373 to close instrument opening...

Page 129: ...Axioskop 2 mot plus may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of your microscope even for a longer per...

Page 130: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus 4 10 B 40 075 e 10 03...

Page 131: ...Axioskop 2 plus ANNEX Axioskop 2 mot plus Overview Carl Zeiss B 40 075 e 10 03 A 1 ANNEX List of abbreviations A 2 List of key words A 4 Patent rights A 8...

Page 132: ...age height setting focus difference in height measurement DC Direct Current DIC Differential Interference Contrast DIN Deutsches Institut f r Normung German standards association DL transmitted light...

Page 133: ...index of the medium nD refractive index of D line sodium nP refractive index of the specimen Ph phase contras PL flatfield R right drive on the right of the mechanical stage SLR Single Lens Reflex SW...

Page 134: ...4 3 6 3 14 Binocular ergo phototube 3 15 Birefringence 3 31 Brightfield 3 8 3 20 3 24 3 27 3 29 C Changing the fuses 4 3 Coarse 3 5 3 11 3 22 Coarse drive 3 7 Color temperature key 3200 K 3 7 Condense...

Page 135: ...odule 2 29 Fluorescence 3 39 Focus positions 1 3 3 21 3 26 Focusing drive 3 5 3 9 3 11 3 13 3 26 Focusing drive 3 7 Front lens 1 3 2 7 2 15 3 8 3 12 3 24 3 26 Function modes 3 18 3 19 G Gear switchove...

Page 136: ...25 3 30 3 40 M Maintenance 4 3 Manual mode 3 18 3 21 Measurement of lengths 3 50 Mechanical stage 2 3 2 22 2 25 3 5 3 7 3 8 Mercury vapor short arc lamp 2 11 3 40 Modules 1 4 1 8 Multi observation equ...

Page 137: ...20 3 22 3 23 3 26 Smoothness 2 24 2 25 3 8 Spares 4 8 Specimen holder 2 3 2 25 Stage carrier 2 15 2 22 2 23 2 7 Stage focusing 1 18 3 9 3 13 3 21 3 22 Start up 2 1 2 3 Switching on 3 18 Switch on swit...

Page 138: ...t rights Axioskop 2 mot plus A 8 B 40 075 e 10 03 Patent rights The instruments instrument components or methods described in this manual are protected by the following patents DE19828548 US6327080 DE...

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