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LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Macro Menu
Carl Zeiss
03/06
B 45-0021 e
4-199
Pixels X
The X pixel resolution of a slice of a single stack can be varied between 4 and
512 Pixels.
Pixels Y
The Y pixel resolution of a slice of a single stack can be varied between 4 and
512 Pixels.
Speed
The scan speed of the
Multiple Stack Scan
mode can be varied between
scan speed 5 and 8 (see
Scan Speed
, page 4-75).
Topography
This checkbox determines whether the result of the
Multiple Stack Scan
is
treated as Multiple intensity stack array or in an reduced XYZ topography
format. If this tickbox is disabled all treatments like for a single confocal image
stack can be applied. If the tickbox is enabled, only topography based
operations could be performed. This
Multiple Stack
experiment needs much
less storage when performing the topography based experiment. But
operations like orthogonal projections and intensity profiles are not any more
available.
Threshold
This intensity threshold has to be chosen before performing a
Multiple Stack
experiment in a topography based format and could not be changed
therafter.
Adjustment functionality:
Find Focus:
Autofocus by fast Z line
Find Gain:
Auto Brightness & Contrast
Adjust scan mirrors:
To XY direction of MOT stage
Adjust spherical
objective error:
On a plane mirror
Macro VBA programming is described in CHAPTER VBA PROGRAMMING FOR LSM of the
printed manual.