OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
3D View Menu
LSM 510 META
DuoScan
4-172
B 45-0021 e
03/06
4.7.4.2 Source
Panel
The image to be processed is selected in the
Source
panel. The currently selected image is
shown in the display box of the image selection
box. Proceed as follows to select a image via the
image selection box:
•
Click on the arrow button. The image selection box will be opened and all the currently loaded images
will be displayed in a minimized form.
•
Click on the required image, which will then appear in the display box of the image selection box and
has been selected for the interpolation procedure.
You can also use the
Click into window
button for image selection.
4.7.4.3 Deconvolution
Panel
The
Deconvolution
panel contains the two tabs
Method
and
PSF
.
(1) Method
tab
The
Method
tab permits selection between the
calculation methods
Nearest Neighbour
,
Inverse
and
Iterative
.
(a) Nearest
Neighbor
The Nearest Neighbor method is the simplest and
fastest algorithm which in principle corresponds to
a 3D sharpness filter.
(b) Inverse
Filter
The regularized inverse filter generally achieves better results than the Nearest Neighbor algorithm. It is
well suited to process several image stacks for a pre-selection of images for the use of the iterative high-
end methods.
(c) Constrained
Iterative
The best image quality is achieved using the Constrained Iterative Maximum Likelihood Algorithm.
Increasing the resolution in the image, especially in the Z-direction, is only possible with this method. Due
to the complex mathematical method, depending on the image size and the PC being used the
calculation can take up to several hours.
In the
Inverse
method, the
Restoration Effect
slider permits the noise-to-signal ratio to be selected
between the settings
Weak
(low noise) and
Strong
(pronounced noise).
Fig. 4-167
Source panel
Fig. 4-168
Method tab
