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LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Process Menu
Carl Zeiss
03/06
B 45-0021 e
4-157
If the analysis is done using the double exponential fit the fitted curve displays the mean of the fitted
values for the two different mobile fractions. The table shows the following additional parameters:
−
The amplitude of the two curves, displayed as one (which equals each part of the mobile fractions)
I1
and
I2
.
−
The fitted parameters
T1
and
T2
(seconds) for each mobile fraction.
−
The rate constant for the exchange of molecules between the bleached region and the surrounding
area
K1
and
K2
(per second) for each mobile fraction.
The raw data of the experiment can be exported for further analysis using the Mean ROI display
mode and, within this dialogue, the table display of the results.
Note that this modeling is also a very basic approach to your experiment. It offers a first hint on
the possible presence of two mobile fractions of the labeled protein within the cell or cell
compartment examined.
Please refer to relevant scientific literature or the website of the EAMNET (http://www.embl.de/eamnet)
for further information on how to set up and perform FRAP experiments. A schematic curve marking the
data points that are calculated performing the Kinetic Analysis is shown in Fig. 4-145. Please note, that
the naming of the data points is not consistent with the information on the website.
Fig. 4-144
Image window displaying the analysis of FRAP data using a double
exponential fit