LSM 510
Routine Mode
LSM 510 META
Application of Examination Methods in …
Carl Zeiss
03/06
B 45-0021 e
6-11
Optical Slice:
The
Optical Slice
window displays the following information:
Black:
Stack Z Size (µm) = intervals x (number of slices - 1)
Optimal Interval = depending on the objective used and the pinhole diameter setting
Red and other colors:
Presentation of the actual data set by the operator helps to optimize stack creation.
For redefining the parameters of a Z Stack, use the
setting functions of the
Mark First/Last
panel.
The optimum stack size (definition of the upper
and lower limits of the stack) is determined by
focus adjustment during a continuous scan.
•
Click on the
XY cont
button.
−
A continuous XY-scan of the set focus
position will be performed.
•
Use the manual focusing drive or the
Stage
and Focus Control
window to focus on the
upper position of the specimen area where the
Z Stack is to start.
•
Click on the
Mark First
button to set the upper
position of the Z Stack.
•
Then focus on the lower specimen area where the recording of the Z Stack is to end.
•
Click on the
Mark Last
button to set this lower position.
•
The
Num Slices
slider enables you to set the number of slices. The limits of the Z Stack remain
constant; the interval is matched accordingly.
The changes made are visualized online in the
graphic display of the
Optical Slice
panel.
•
Click on the
Start
button to start the recording
of the Z Stack.
In case the upper and lower limits of the stack
have been switched round, automatic matching
will be performed by the software, since the stage
of the Axio Imager.Z1 always moves from bottom
to top and the nosepiece of the Axiovert 200 M
always moves from top to bottom.
Fig. 6-14
Mark First/Last panel
Fig. 6-15
Optical Slice panel
