OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Macro Menu
LSM 510 META
DuoScan
4-206
B 45-0021 e
03/06
Acquisition Action Blocks
Loads a Beam Path Configuration form the Configuration list. Use the pull
down menu in the
Property
/
Value
list to select the Configuration that should
be used for imaging. Changing between Single Track and Multi Track allows to
chose between the different Configuration lists. Multi Track also lists tracks that
are used for Emission Fingerprinting or Online Fingerprinting. Depending on the
type of track different settings can be loaded optionally. If they are not loaded
and the parameters list of the Scan Parameter block does not offer them as
being editable, for example detector gain and offset, default values will be
used.
The list of properties lists all parameters that can be adjusted for the scan
procedure. The value for each parameter can be typed in individually. Clicking
the
Read Back
button will take over all current settings from the main
software. The check boxes next to the parameters can be marked individually
or, clicking
Use all
or
Use none
they can be all checked or unchecked with
one click. Only the parameters with a mark in the check box will be taken into
account and adjusted when the Scan Parameter block is set in the program
flow column. This allows to change only some of the parameters, for example
the zoom, at a certain position within the program flow as for each block the
check boxes can be set.
This block performs the scanning of the sample according to the settings
chosen with Beam Path and Scan Parameters. It can be linked to the Data flow
column. If no connection to the data flow is made (for example: Display Image)
the result of the scan will not be displayed.
The parameters for bleaching the sample can be loaded from the list of bleach
settings. Bleach settings are assembled and stored in the Bleach control window
of the main software.
This block performs the bleaching of the sample according to the bleach
settings that have been defined and stored in the Bleach control of the main
software. Use the pull down menu to select the bleach setting from the list.
The list of properties lists all parameters that can be adjusted for the acquisition
of the time series.The value for each parameter can be typed in individually.
Clicking the
Read Back
button will take over all current settings from the main
software. The check boxes next to the parameters can be marked individually
or, clicking
Use all
or
Use none
they can be all checked or unchecked with
one click. Only the parameters with a mark in the check box will be taken into
account and adjusted when the Time series block is set in the program flow
column. This allows to change only some of the parameters, for example the
used trigger, at a certain position within the program flow as for each block the
check boxes can be set. This block can be connected to the data flow column.
