LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Macro Menu
Carl Zeiss
03/06
B 45-0021 e
4-185
4.8.4.3
Profile Macro – Profile.lvb
This macro permits the gray values of a line created in the scanning image to be determined pixel by
pixel.
•
Click on the
Profile
button in the
Macro
subordinate toolbar.
−
An XY scanning image of the specimen is recorded and displayed. The
Profile
window is shown on
the screen.
•
Then click and hold down the mouse button to draw a line in the scanning image for which the gray
values shall be determined.
−
The current numbers of the pixels of the created line to which the relevant gray value is assigned
now appear in the
Profile
window.
−
At the same time, the distance of the created line is displayed in µm for checking.
−
Any required number of lines can be defined in the image. The previous line is deleted.
•
A click on
Cancel
will end the macro.
4.8.4.4
Bleach Macro – Bleach.lvb
This macro permits the specimen to be excited with the laser as required along a line created in the
scanning image. Useful for systems LSM 5 PASCAL which do not have the Bleach functionality in the
main software.
•
Click on the
Bleach
button in the
Macro
subordinate toolbar.
−
An XY scanning image of the specimen is recorded and displayed. The
Spot Scan
window is
shown on the screen.
•
Click on the
Select Excitation Line
button.
•
Create a free-hand line (spline) in the scanning image over the area to be excited by clicking and
holding down the mouse button.
•
Then determine the duration of the excitation by moving the Exposure Time slider.
•
Click on the
Excite
button to trigger the excitation procedure.
•
A click on
Exit
will end the macro.
