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In the
Sum
method, the pixel values of all scans are only added up, without a mean value being
calculated.
•
Select the Number for averaging.
Continuous
averaging is possible in the
Frame
mode. In this case a
Finish
button for ending continuous
averaging is displayed instead of the
Cont.
button.
Adjusting the pinhole
•
Select
Channels
in the
Scan Control
window.
•
Set the
Pinhole
size to
1
(Airy unit) for best
compromise between depth discrimination and
efficiency.
Pinhole adjustment changes the
Optical Slice
.
When collecting multi channel images, adjust the
pinholes so that each channel has the same
Optical Size
. This is important for colocalization
studies.
Image acquisition
Once you have set up your parameter as defined in
the above section, you can acquire a frame image
of your specimen.
•
Use one of the
Find
,
Fast XY
,
Single
or
Cont
.
buttons to start the scanning procedure and
acquire an image.
Scanned images are shown in separate windows.
•
Click on the
Stop
button to stop the current
scan procedure if necessary.
Select
Find
for automatically pre-
adjustment of detector sensitivity.
Select
Fast
for continuous fast
scanning – useful for finding and
changing the focus.
Select
Single
for recording a single
image.
Select
Cont.
for continuous scanning
with the selected scan speed.
Select
Stop
for stopping the current
scan procedure.
Fig. 17
Scan Control window, Channel settings
Fig. 18
Image window
