OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-86
B 45-0021 e
03/06
•
Moving the green line (current slice) enables you to change the current focus position (moving the
stage or nosepiece in the process). The stack limits are also changed, while interval and Num Slice
remain unchanged.
•
Shifting one of the red lines enables you to change the stack size; in that case, the interval size is
matched, and the Num Slice remains constant.
−
Changing the values of
Num Slice
,
Interval
and
Current Slice
in the
Z Sectioning
tab will, of
course, also change the positions of the red and green lines in the
Image Display
window.
•
A click on the
Start
button will start the recording of the Z Stack.
−
The settings of the entire
Scan Control
window (
Mode
,
Channels,
Z settings
) will be used when
the stack is produced.
In Frame mode the stack is acquired as a stack of images in xyz. In Line mode the stack is
displayed by a xz image. Continuous imaging of the xz stack is also possible.
Fig. 4-66
Scan Control window and Image Display window