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OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Maintain Menu
LSM 510 META
DuoScan
4-228
B 45-0021 e
03/06
(2) Calibration
conditions
•
The microscope stand has to be placed totally vibration-free.
−
Note, that even power units on the granite plate or inappropriate situated cables can cause
vibrations.
•
Before the calibration process is started, the system has to be in operation for at least one hour
(better: two hours).
−
Otherwise, the tuning results will be incorrect and the forward / backward image contents do not
match with each other.
•
The longest available laser wavelength of the system has to be applied.
•
A 80/20 neutral beam splitter and a
None
position in the emission filter wheel of either Channel 1 or
2 has to be used in the
Scan Configuration
window.
•
A Plan-Neofluar 10x/0.3 or Epiplan Neofluar 10x/0.3 objective lens has to be used.
•
A special sample (see Fig. 4-225) with two identical but 90 degrees rotated gratings (one for each scan
direction) has to be used as a calibration standard.
•
The pinhole has to be completely opened.
•
A dynamic range of 8 bit has to be used.
(3) Calibration
procedure
•
It is advantageous to perform the electrical calibration before starting the optical calibration process.
For a first scan of the calibration standard start with scan speed 12 and zoom 3.6 (unidirectional).
•
Focus on the calibration standard and adapt the dynamic range of the detector on the sample.
−
Optimize
Detector Gain
and
Ampl. Offset
values in the
Channels
sheet of the
Scan Control
window by means of the
Range Indicator
mode.
Fig. 4-225
Calibration standard