OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Display and Analysis of Images
LSM 510 META
DuoScan
4-280
B 45-0021 e
03/06
•
Specify the thresholds either with the
Threshold low
and
Threshold high
sliders, or with the
▲▼
buttons.
4.13.20.3 Colocalisation
Function
The
Colocalisation
function permits interactive analysis of two channels of an image by computing a
scatter diagram (colocalisation).
•
Click on the
Colocalisation
button. The scatter diagram is created and displayed beside the image.
•
Click on the
xy
button of the
Display
toolbar if you want to return to the original image.
How a scatter diagram is generated
All pixels having the same positions in both images are considered a pair. Of every pair of pixels (P1, P2)
from the two source images, the intensity level of pixel P1 is interpreted as X coordinate, and that of pixel
P2 as Y coordinate of the scatter diagram. The value of the pixel thus addressed is increased by one every
time, up to the maximum number of pixels used. This way, each pixel of the scatter diagram is a value
that shows how often a particular pair of pixels has occurred.
Differences between the images cause irregular spots in the scatter diagram.
Identical images produce a clean diagonal line running from bottom left to top right, because only pixel
pairs (0,0), (1,1), (2,2) with the same intensity can occur. Differences between the images cause an
irregular distribution in the scatter diagram.
Fig. 4-274
Image Display window, Colocalization display
