OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Image Optimization
LSM 510 META
DuoScan
4-346
B 45-0021 e
03/06
•
Click on the
icon, activate the
543 nm
laser line and click on
Line Active
. If
required, deactivate other laser lines which are
not needed.
•
Use the
Transmission
slider to set the laser
intensity to approx. 30 % at first.
The
Beam Path and Channel Assignment
panel displays the current configuration loaded.
The set laser intensity must be subsequently optimized for the current situation via the
Transmission
slider.
For overlaying fluorescence and transmitted-light
images, click on the
ChD
(Transmission) button in
the
Beam Path and Channel Assignment
panel.
The transmitted light PMT photomultiplier will be
activated.
Of course, all other transmitted light applications
like
−
phase contrast
−
differential interference contrast (DIC)
−
polarization contrast (Pol)
−
darkfield
can also be performed.
For the generation of images in reflection,
the main dichroic beam splitter must be a neutral-
density filter.
Standard equipment contains a neutral-density
filter with a division ratio of 80 to 20 % (at
543 nm).
Fig. 4-323
Excitation panel
Fig. 4-324
Configuration Control window