LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Acquire Menu
Carl Zeiss
03/06
B 45-0021 e
4-77
−
A click on the center button will recenter the scan area to the field of view.
−
Clicking, holding and drawing the rectangle with the mouse permits the scan area to be moved
directly within the field of view.
−
Recommended setting to start with: Offset X = 0, Y = 0
During the scan procedure, the functions
Objective change
,
Speed
,
Scan Corr
,
Zoom
,
Rotation
and
Offset
can be influenced online.
By clicking on the
Reset
button the scan zoom is set to 1 and the XY offsets are set to the zero position
and the ratio angle is set to 0°.
(2) Channels
If the
Channels
button is activated, the
Channel
Settings
and
Excitation of Track ...
panels are
displayed in the
Scan Control
window.
Channel Settings
panel
In the
Channel Settings
panel, the channels (incl.
META channels if present and ratio channels)
defined in the
Configuration Control
window are
listed track by track as selectable buttons.
Depending on the selected
Channels b
utton (e.g.
ChS1-T1
), the currently used settings of Pinhole,
Detector Gain, Amplifier Offset and Amplifier Gain
are displayed.
•
The slider near
Pinhole
enables you to change
the pinhole diameter of the relevant channel.
−
The pinhole diameter is indicated in
µm
,
Optical Slice
and
Airy Units
. The Airy value
depends on the aperture of the objective,
excitations and the emission wavelength.
−
A small pinhole diameter will increase the
depth of focus, but reduce the light intensity
received by the PMT photomultiplier.
−
When you vary the pinhole diameter, an
Optical Slice value is displayed. For optimum
depth resolution, Airy values should be small,
but in fluorescence applications not below
1.0 to keep the intensity loss within a
reasonable limit.
−
A click on the
1
button sets the pinhole to a
diameter of 1 Airy unit. A click on the
Max
button sets the pinhole diameter to the
maximum.
Fig. 4-56
Scan Control window – Channels
