LSM 510
Routine Mode
LSM 510 META
Application of Examination Methods in …
Carl Zeiss
03/06
B 45-0021 e
6-13
•
Then click on the
Range
button.
−
The XZ-scan will be performed and displayed in the image window. At the same time, the position
of the current slice is shown with a green line and the positions of the first and last slice with two
red lines.
•
Moving the green line (current slice) enables you to change the current focus position (moving the
stage or nosepiece in the process). If the green line (Current Slice) is shifted after the creation of
Range
, the focus position will change (the piezo fine focusing stage remains in the center position).
•
Shifting one of the red lines enables you to change the stack size. The red lines (stack limits) can only
be changed symmetrically to the Current-Slice position within the piezo fine focusing stage work
range.
Since the piezo fine focusing stage moves from bottom to top during the creation of the Z Stack,
top and bottom of the Axiovert 200 M have been switched round.
•
A click on the
Start
button will start the recording of the Z Stack.
•
When recording of the Z Stack is completed, click on
Next
.
−
The
Routine Mode - Image Save, Print & Export
window appears.
Fig. 6-17
Image window
Red upper Z range limit
Green cutline (current slice)
Red lower Z range limit
