OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-66
B 45-0021 e
03/06
4.5.3.8
Settings in the Lambda Mode
The
Lambda Mode
settings are only available if
the LSM 510 contains the META detector. With
this spectral module the overall emission from the
sample is directed onto a wavelength-dispersive
element and is imaged on a 32 channel detector.
All 32 photomultipliers of the detector cover a
spectral width of approximately 340 nm, a single
PMT covers a spectral range of 10.7 nm. In the
Lambda Mode,
images, image stacks or time
series can be recorded in a wavelength selective
way.
This new experiment is called Lambda Stack. For
the acquisition of a Lambda Stack, the
fluorescence signal of 8 PMTs out of the 32 can be
read out at once. The settings of the beam path
for the
Lambda Mode
scanning procedure with
regard to the main dichroic beam splitter and the
META detector settings are performed in the
Beam Path and Channel Assignment
panel.
•
Click on the
Config
Button in the
Acquire
Subordinate toolbar of the main menu.
−
The
Configuration Control
window opens.
•
Click on the
Lambda Mode
button, unless it
has already been activated.
−
The
Beam Path
and
Channel Assignment
panel for the
Lambda Mode
is opened.
(1)
Beam Path and Channel Assignment panel
The
Beam Path and Channel Assignment
panel displays the configuration of laser lines, the main
(dichroic) beam splitter and the spectral range of the META detector to be covered.
You can change the settings of this panel using the following function elements:
Beam Path configuration
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider). Open the
Laser Control
window via the
Laser
button. The active laser lines are automatically displayed in the wavelength color
bar.
Selection of the main dichroic beam splitter (HFT) through selection from the
relevant list box.
META detector
slider Definition of number of PMTs out of the 32 PMT Detectors to fit the required
spectral range.
Fig. 4-47
Configuration Control window;
Lambda Mode activated
