ThermoFisher Scientific 912A0604 Manual Download

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Procedure: Installation

Title: Installing the iN10/iN10MX
Revision: 09
Procedure # Install-001
ECCN: EAR99

This document contains confidential or proprietary information of Thermo Fisher Scientific. Neither this

document nor the information therein is to be reproduced, distributed, used or disclosed, either in whole or

in part, except as specifically authorized by Thermo Fisher Scientific.

8/3/2020

Page 1 of 61

iN10/iN10MX

Part number:

912A0604/912A0605/912A0804/912A0805

Part description:

iN10/iN10MX Integrated Infrared Microscope

Tools required:



Scissor



Short handled medium Phillips screwdriver



Long handled medium Phillips screwdriver



Flat blade screwdriver



5/64” balldriver or L-wrench (array alignment)



Needle nose pliers (iZ10 interface installation)



Pliers (iZ10 installation)



3/16” L-wrench (iZ10 installation)



5/32” balldriver (iZ10 installation)



Laboratory grade powder-free gloves



ATR Crystal alignment procedure



Service test slide



100um Pinhole slide

Estimated time required:

Approximately 4 -12 hours depending on

configuration.

Notes:

N/A

Summary of Contents for 912A0604

Page 1: ...MX Part number 912A0604 912A0605 912A0804 912A0805 Part description iN10 iN10MX Integrated Infrared Microscope Tools required Scissor Short handled medium Phillips screwdriver Long handled medium Phil...

Page 2: ...estraint and install the Dewar fill extender Deleted step to verify video calibration parameters from Sync Settings as it is not necessary Modified steps as necessary Modified mapping steps to contact...

Page 3: ...except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 3 of 61 Important Prior to performing any replacement procedure you must read the Nicolet iN10 Safety Guide If you do not ha...

Page 4: ...th validation certification complete this installation procedure before performing ValPro 2 Release bands and unwrap the system The system is shipped with the main system package and the computer pack...

Page 5: ...ther in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 5 of 61 4 Open the main system shipping box Using a scissor cut and remove the plastic bands that h...

Page 6: ...distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 6 of 61 7 Remove the accessory box 8 Open the Accessory box 9 Remo...

Page 7: ...standard the slide on ATR kit optional and the ValPro Standards optional Set the boxes aside and remove the X Y stage package 11 Verify all components are present per the packing list located in the...

Page 8: ...61 14 Remove the plastic bag Cut and remove the plastic bag and discard the shipping desiccant packages Carefully remove the bubble wrap from the area between the objective and condenser 15 Remove shi...

Page 9: ...ap the motorized X Y stage and set the stage aside 18 Remove the stage cable and sample insert Remove the X Y stage cable and standard sample insert from under the packing foam and set aside Continue...

Page 10: ...ue with step 26 If you have an imaging option or an iZ10 option continue with step 22 22 Remove the main cover of the iN10 Using a short handled Phillips screwdriver remove the 5 screws from under the...

Page 11: ...the iZ10 If an iZ10 was not ordered continue with step 25 24 1 Remove the iN10 base cover Using a short handled Phillips screwdriver remove the four screws from the base cover of the iN10 and set the...

Page 12: ...12 of 61 24 3 Remove the AEM flipper shipping restraint Using a 5 32 balldriver remove the screw and shipping restraint from the AEM flipper assembly Store the screw and shipping restraint with the cu...

Page 13: ...ntific 8 3 2020 Page 13 of 61 24 6 Remove the purge plugs from the iZ10 Using needle nose pliers remove the 4 purge plugs from the iZ10 There 2 plugs on the left edge as depicted below 1 in the sample...

Page 14: ...fic 8 3 2020 Page 14 of 61 24 8 Attach the interface plate to the iN10 Tip the iZ10 up from the right side so the interface plate tips down and align the channel with the pin under the left side of th...

Page 15: ...m hole plug from the left side of the cover of the iZ10 if applicable Store the plug with the customer items 24 12 Reinstall the iZ10 cover 24 13 Reinstall the base cover on the iN10 Using the screws...

Page 16: ...s on the outer ring to fully install seat the lens assembly 24 17 Re install the main cover Using the screws removed in step 22 secure the main cover 24 18 Install the purge baffle between the iN10 an...

Page 17: ...urn the quick release knob on the X Y stage counter clockwise 3 4 turn to set the quick release for installation 26 1 Install the X Y stage on to Z stage dovetail mount Slide the back of the stage on...

Page 18: ...power connector to the electronics module at the rear of the iN10 Plug the power cord into the power outlet Turn the power on to the iN10 28 Connect computer components Connect the monitor s mouse key...

Page 19: ...Microscope is set to Nicolet iN10 click Save and then close Omnic Picta 32 2 Change the Correction Factor From C My Documents OMNIC open the OmnicSettings ini file Change the UseCorrection factor fro...

Page 20: ...e Control screen From the Window drop down menu choose Stage Control 32 6 Initialize the X Y Stage Click Initialize XY to begin initialization 32 7 Check the centering of the stage At the right front...

Page 21: ...21 of 61 32 9 Set origin From the Window drop down menu choose User Configuration Click Set XY Stage Origin This resets the home position 32 10 Verify alignment Choose the Stage Control tab and click...

Page 22: ...tick and use the Z stage focus control to lower the stage so it is approximately 1 from the bottom of the objective 32 14 Prepare to adjust the video properties Place a white business card on the cent...

Page 23: ...Fisher Scientific Neither this document nor the information therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Sci...

Page 24: ...ormation therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 24 of 61 32 15 Set video prope...

Page 25: ...tributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 25 of 61 32 15 1 Turn off all Auto gains and then select Advanced If a...

Page 26: ...hole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 26 of 61 32 15 2 Set the White Balance Select the White Balance tab and from the White Balance mode drop dow...

Page 27: ...61 32 15 3 Save the video properties Click the Load Save 1 tab and choose Save As 2 When the dialog box opens type in Default 3 and choose OK 4 Default will appear in the white screen to the left Clic...

Page 28: ...part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 28 of 61 33 Configure the optional components 33 1 Configure the mechanical joystick Select the User Configuration tab...

Page 29: ...joystick cable Connect the joystick to the electronics module at the rear of the iN10 33 2 Turn the power on to the iN10 Wait until the blue power LED s stop blinking and then proceed with step 33 3...

Page 30: ...ntific 8 3 2020 Page 30 of 61 33 5 Configure ATR From the Window drop down menu choose User Configuration Check ATR is installed If you do not have an ATR continue with step 34 34 Verify aperture cent...

Page 31: ...blinking and then continue with step 34 2 34 2 Open the iN10 Configure screen Launch DDiag and from the Protected Content drop down menu choose Configure iN10 34 3 Enter aperture calibration paramete...

Page 32: ...menu choose Motor Control From the Select Stepper 1 box click the arrow to the right 2 of the centered drop down menu and then use the scroll bar 3 to find and select CM_DUR_DEV_CONDENSER In the Steps...

Page 33: ...rwise continue with step 38 Allow at least 20 minutes for each detector to stabilize after cooling before running tests on the cooled detectors Note The following step selects and uses the TGS detecto...

Page 34: ...s the condenser stage back to its original position after it performs an alignment and the condenser may no longer be at the optimal focal point From the OMNIC Picta toolbar click the Slide View icon...

Page 35: ...tor Control Using the arrows to the right of each dialog box X Y micrometers set the aperture to 100 x 100 42 Run the system performance test in transmission mode From the Diagnostics drop down menu c...

Page 36: ...ic Neither this document nor the information therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020...

Page 37: ...ick the MCT detector icon and then click Run Test When the Select Object and Final Format window appears confirm that the reflectance measurement box is NOT checked click OK to close the window and th...

Page 38: ...ld mirror If using a motorized X Y stage use the X Y and Z controls of the joystick if using a manual X Y stage use the X Y stage knobs and the Z control of the joystick to move the gold mirror on the...

Page 39: ...mance test diagnostic window and continue with step 42 8 42 8 Close OMNIC and the iN10 diagnostics Close OMNIC close all individual windows in DDiag and then close DDiag Note The following steps are r...

Page 40: ...tage Aperture and Display section Change the Aperture width and height to 100 x 100 NOTE Highlight and change the value and then use the tab key to exit the dialog box and initiate the change Make sur...

Page 41: ...sclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 41 of 61 42 12 Center the pinhole From the tool bar click the Map View icon and then choo...

Page 42: ...2020 Page 42 of 61 42 13 Set map parameter From the View and Collect Experiment Setup tab scroll down to the Stage Aperture and Display section and change the following parameter Aperture 10 x 10 for...

Page 43: ...61 42 16 Define the background point Turn the lower illuminator off if it is on and using the joystick move to the center of the open hole on the pinhole slide Click the Background Point Tool and the...

Page 44: ...42 19 Blend 2 D IR and Video Scroll down to the Analyze menu on the left and choose Blend 2 D and Video 42 20 If the IR and Video overlap exactly or the IR is displaced 5 microns or less from the vid...

Page 45: ...er being careful not to disturb the pinhole alignment or bump any optical components 42 23 Adjust the single point dichroic Depending on the location of the IR make a small adjustment to the appropria...

Page 46: ...rt except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 46 of 61 43 2 Center and focus the pinhole Use the Stage Tool to click the center of the pinhole to center re center the...

Page 47: ...ientific 8 3 2020 Page 47 of 61 43 5 Collect map From the lower right side of the screen click the Collect icon and enter a title for the map The map collect will start 43 6 Compare 2 D IR image to vi...

Page 48: ...tector mirror If the IR is shifted in any direction between 5 and 25 microns from the video image use a 5 64 balldriver to adjust the appropriate screw on the first array detector mirror to adjust the...

Page 49: ...lect menu turn the Imaging zoom optics on by checking the Imaging zoom optics box as indicated below 44 2 Collect a pinhole map Re center the pinhole in the FOV if necessary and shrink the area map si...

Page 50: ...ic window again and choose Delete Background Point 46 Verify ATR alignment if applicable If the system does not have an ATR continue with step 48 46 1 Select Reflection Mode From the OMNIC Picta View...

Page 51: ...l is roughly centered in the crystal holder equal gap around the entire circumference between the slide and brass insert If the gap is not equal adjust the alignment and plunger screws as necessary to...

Page 52: ...us for maximum throughput From the preview window turn Preview on While observing the throughput use your fingers to slowly rotate the crystal clockwise in the holder to maximize throughput as display...

Page 53: ...til it clicks in the first detent and is out of the IR beampath 46 13 Insert one of the polycarbonate slides from the customer tool kit onto the ATR sample holder 46 14 Change system parameters From t...

Page 54: ...earance between the polycarbonate slide and ATR crystal tip fully insert the slide back into the dovetail until it hits the hard stop Note If you have an original ATR sample holder rotate the holder s...

Page 55: ...the first detent and is out of the beampath 46 21 Focus Z Stage Without moving the X Y position of the stage bring the Z Stage into focus and view the location of the crystal footprint It must be cent...

Page 56: ...6 of 61 46 22 Center the ATR crystal Using two 5 64 balldrivers adjust the two alignment screws to move the crystal in the direction necessary to center the footprint on the crosshairs 46 23 Verify cr...

Page 57: ...as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page 57 of 61 48 Validate the iZ10 if applicable otherwise skip to step 51 Close OMNIC Picta and launch OMNIC From the Collect drop dow...

Page 58: ...authorized by Thermo Fisher Scientific 8 3 2020 Page 58 of 61 48 2 Choose the detector In the Advanced Diagnostics window click the Performance Test button and then click the icon for the iZ10 TGS de...

Page 59: ...ualification test to be completed and click OK to close the window Click Exit to close the ValPro window 51 3 Start the ValPro test Click the ValPro icon and choose the microscope EP test from the dro...

Page 60: ...ither this document nor the information therein is to be reproduced distributed used or disclosed either in whole or in part except as specifically authorized by Thermo Fisher Scientific 8 3 2020 Page...

Page 61: ...the Flash ROM on the core card OMNIC_MC ini information and other information relevant to the system configuration and status Close the c Program Files omnic window when complete 56 Open and print a c...

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