Siemens ADVIA 2120, Руководство оператора, Страница: 378 / 477

Regulatory Information 9-37
1. If the Standby indicator is lit, press the Standby button to put the system into
Ready to Run mode.
2. Using the Order Entry tab, create a workorder for each CSF sample
preparation to be analyzed. Include sample physical characteristics in the
Ocom
field and, if necessary, the dilution factor in the
Dil
field. Enter
CSF
as
the sample type and select the
Tab
key in order to see the correct menu of
tests.
3. At the Routine Operations menu, select the
Manual Sample ID
tab.
4. Select the
Cerebrospinal Fluid
button.
5. Select
Initialize
to wash the pathways and obtain a flow cell background
count. After the system has completed the initialization cycle and the sample
aspiration light is green, verify that background counts in the flow cell
pathway are within acceptable limits. If background counts are not
acceptable, perform one aspiration of a 25% bleach solution once, followed
by two aspirations of distilled water or saline. The recommended values for
these limits are:
CSF R Count = 0
CSF PHA WBC = 0
CSF PHA Total <=10
If background counts are not acceptable, perform one aspiration of a 25%
bleach solution once, followed by two aspirations of distilled water or saline.
NOTE:
The
CSF Wash
cycle rinses the sample pathways without performing
a background count. The
CSF Refresh
cycle refreshes the flow cell
background count without rinsing the sample pathways. The background
counts are stored in the startup log.
6. Select
Primer
as the Sample Type and select
OK
. Aspirate a saline primer to
verify that background counts in the sample pathway are within acceptable
limits. Make sure that
Primer
displays on the status line before aspiration.
7. If background counts are acceptable, proceed to step 8. If not, perform one
aspiration of a 25% bleach solution followed by three aspirations of distilled
water or saline, and repeat steps 5 and 6.
8. Select the correct sample type and then enter the sample or control
information in the
Manual Sample ID
box or scan the barcode on the sample
tube. Do not use sample barcode labels with selectivities encoded in them to
enter sample identifications.
CAUTION:
Make sure the correct sample ID and sample
type appears on the status line before aspirating a sample
using the manual open-tube sampler.
9. After appropriate incubation, mix the sample again, either by inverting it 5-
10 times or by vortexing it for 5 seconds immediately before aspiration on
the ADVIA 120 Hematology System. Cells in prepared CSF samples will
settle quickly due to low viscosity.