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Methods 8-3
Basophil / Lobularity Method
Cytochemical Reactions
The basophil/lobularity cytochemical reactions consist of two steps:
Step 1
Red blood cells and platelets are lysed using the ADVIA 2120/2120i
BASO reagent.
Step 2
All white blood cells
except
basophils are stripped of their cytoplasm
using the
ADVIA 2120/2120i BASO reagent and the increased temperature in the
reaction chamber.
The stripped white cells can now be categorized as mononuclear or
polymorphonuclear cells based on the shape and complexity of their cell nuclei.
The intact basophils can be easily distinguished from the smaller cell nuclei.
Basophil / Lobularity Temperature Control
Temperature control is critical to the cell-stripping process. The baso reaction
chamber temperature, which is automatically monitored by the analyzer, must be
between 32 °C and 34 °C.
If you want to check the baso reaction chamber temperature:
At the Utilities menu, select
Analyzer Status
.
If you need to adjust the baso reaction chamber temperature:
1. At the Special Procedures menu, select
Adjust Lamp / Temp
.
2. Select
Baso Temperature
.
ADVIA 2120/2120i BASO Reagent
NOTE:
For more detailed information on the contents of ADVIA 2120/2120i
BASO reagent, please see Chapter 8.
The ADVIA 2120/2120i BASO reagent contains phthalic acid and a surfactant
that lyses the red cells, platelets, and the cytoplasm of all white cell types except
basophils.
ADVIA 2120/2120i SHEATH / RINSE
NOTE:
For more detailed information on the contents of ADVIA 2120/2120i
SHEATH/RINSE, please see Chapter 8
ADVIA 2120/2120i SHEATH/RINSE has several important functions:
Optical sheath for Baso, RBC / Platelet, and Reticulocyte Methods
•
Constricts the sample stream so that only one cell at a time can pass through
the viewing area