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ZEISS
Center Screen Area / Image Containers - Display and Image Analysis
ELYRA 7
362
000000-2262-999
03/2019 V_02
SMLM-Drift
: Setting parameters for drift correction using fiducial markers or models and performing
drift correction. Viewing fiducials and corrected drift.
SMLM-Group
: Setting parameters for grouping and performing grouping of identified peaks to one
object.
SMLM-Statistics
: Viewing Molecule table, statistic plots (histograms & scatterograms) and peak tracker
(only available for
Online Processing
).
SMLM-Filter
: Setting and executing filters including all parameters of the molecule table.
SMLM-Render
: Setting pixel resolution, display and rendering of data.
There are also
general control view
tabs
available (Fig. 582), which functions have been
described in section
Dimensions
,
Display
and
Graphics
, but that
have special functions in relation to the
SMLM
view tab.
6.18.1
Dimensions View Control Tab
Time
,
Zoom
,
Tools
and
Channels
as well as
Reuse
,
Crop
,
Positions
and
Stage
are general functions
that have been described in section
If the data set like in 3D has a z dimension, the dimension will be displayed as Z-Position and with the
Z-Position slider you can scroll through the data set (Fig. 583).
Please note that the spinbox display shows the number of the current slice. You can view the
absolute Z-position in µm if you position the cursor on top of the slider button (Fig. 583). The
display box is in the form "current slice
#
(total slice number), position
#
in µm". The position
number in
SMLM-Filter
is given in nm. Hence the info box the slice
#
of the Z-Position slider of
Dimensions can be linked to the position
#
of the
Position Z
slider of
SMLM-Filter
Care has to be exerted when relating relative slice numbers to absolute z positions. If z positions
are restricted for absolute positions using the
Positioin Z
tool of
SMLM-Filter
, than the start slice
is reset to 1 for the Z-Position and hence the slice number corresponds to the absolute position plus
offset from a previous filtering. We recommend therefore that before resetting z-postions the
Position Z
filter tool is de-activated or using the
Position Z
histogram to find a useful z-range.
Fig. 582
General view control tabs for SMLM
Fig. 583
Z-Position slider of the Dimensions tab with curser on the
slider tool
Fig. 584
Position Z slider of the SMLM-Filter tab with minimum value
set to a position corresponding to slice 60 of Fig. 583