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ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
57
(1) Conventional setting of the microscope
Axio Observer
The Recording of microscope settings works as
described for the microscope Axio Imager.
For the conventional setting of the Axio Observer,
proceed as follows:
•
Click the
Locate
tab in the
Main
toolbar.
•
Place specimen on microscope stage.
−
The cover slip must be facing down.
•
In the
Objective
list box, select the required
objective.
•
Use the focusing drive to focus the required
specimen plane.
•
Select specimen detail by moving the stage in
X and Y via the XY stage fine motion control.
(2) Microscope settings on Axio Observer
for transmitted-light observation
•
Click on the
Transmitted light
button. Click
the
On
button in the
Transmitted Light
panel
and set the transmitted light intensity via the
slider or click on
3200 K
.
•
Click on the
Condenser
button and set the aperture via the slider in the
Condenser
panel. Set the
filter in the
Filter
selection box.
•
Click on the
Objective
button and select the objective by clicking on it.
•
Click on the
Reflector
button and select
None
.
•
Make sure there is no illumination from the reflected-light.
(3) Microscope settings on Axio Observer for reflected-light observation (Epi-fluorescence)
•
Turn on the HBO 100 power supply switch.
•
Click on the
Reflected Light
button and set the shutter to
Open
.
•
Click on the
Reflector
button and select the desired filter set by clicking on it.
•
The filter is automatically moved into the beam path to enable observation in epi-fluorescence.
•
Click on the
Objective
button and select the objective.
•
Make sure that the illumination from the transmitted-light is switched off.
Fig. 60
Axio Observer controls in the
Microscope Control tool