ZEISS
Left Tool Area and Hardware Control Tools
ELYRA 7
146
000000-2262-999
03/2019 V_02
5.2.21.3
Lowering Objective when Moving the Stage
Some acquisition experiments require the objective lens to be lowered (or raised depending on the
microscope) before moving the scanning stage to the next position (e.g. required if certain plate formats
are used).
With the tool Positions, this is possible when activating
check box.
This feature is available to
Position List
and
Sample Carrier
if
Show all
is active.
5.2.21.4
Scan Overview Image
Pressing the
Scan overview Image…
button
opens the
Scan overview Image
interactive
window (Fig. 241). Here you can define the
number of
Tiles
by using the
Horizontal
and
Vertical
Input boxes (the extension of the scanned
area will be displayed in
Pixel
and
Size (
µ
m)
units), the objective from the
Objective
drop
down menu and a potential zoom factor via the
Zoom
slider or Zoom input box with which to scan
an overview image. For widefield modes, only the
zoomed in part of the camera chip will be
displayed. Changing
Objective
and
Zoom
will
change the size, but leaves
Pixels
unchanged. This
overview image can then be used to navigate in
the sample (i.e. define positions for imaging).
Scan
starts the tile scan.
Cancel
closes the window
without further action.
Fig. 241
Scan panel