![background image](http://html1.mh-extra.com/html/zeiss/elyra-7/elyra-7_operating-manual_3332235079.webp)
ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
75
5.2.5.4
Tube Lens Selection
The correct tube lens is automatically chosen for the
corresponding imaging mode, but can be altered sub
sequentially. The tube lens can be selected via a graphical pop-
up menu of the
Tube lens
button (Fig. 93). For
WF
imaging
mode,
Lens 1x
is appropriate, for
Laser WF, SIM Apotome
and
SIM
imaging modes
Lens 1.6x
should be used.
5.2.5.5
Filter Cube Selection
The filter cube can be selected from the reflector turret via a
graphical pop-up menu when the
Filter cube
button is
pressed (Fig. 94).
There are six positions for the filter cube. 2 positions (positions
1 and 2) are for push and click filters.
Transmitted Light
(LSM/TL filter cube) is needed for LSM and Transmitted Light
imaging and must be assigned to position 1 as this position will
be automatically selected if Transmitted lightillumination is
selected. The other position (position 2) can be equipped with
a cube that is suitable for widefield excitation and observation
through the eyepieces.
Positions 3-6 hold calibrated filter cubes, which are used for
widefield laser illumination in Lattice
SIM, SIM Apotome
and
Laser WF
imaging modes. They are permanently mounted in
the turret and hence to use other filters need exchange of the
whole filter turret.
5.2.5.6
Objective Selection
The objective can be selected via the graphical
pop-up menu when pressing the
Objective
button
(Fig. 95).
Those objective, which are suited for a certain
imaging mode are highlighted, but all objectives
can be selected.
Please note that for SIM the Plan-
Apochromat 63x / 1.4 oil or the
α
-Plan
Apochromat 63x / 1.4 oil and for TIRF the
α
-
Plan Apochromat 63x / 1.4 oil (for sCMOS)
and the
α−
Plan-Apochromat 100x / 1.46 oil
(for EM-CCD) are the objectives of choice.
For Stage and Focus control please refer to
section Acquisition Parameter – Stage and
section Acquisition Parameter – Focus.
Fig. 93
Tube lens selection
Fig. 94
Filter cube selection
Fig. 95
Objective selection – for WF, SIM
and Laser WF imaging mode