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CHAPTER 3 - ADDITIONAL SOFTWARE TOOLS
ZEISS
System Maintenance Tool
ELYRA 7
2
000000-2262-999
03/2019 V_02
1
System Maintenance Tool
With a calibration objective and correct system configuration, the System Maintenance Tool allows
convenient self-adjustment of LSM.
The optical beampath, relative pinhole position and scanner adjustment can be set and checked
automatically with this tool. In systems that combine different imaging devices (e.g. ELYRA), the System
Maintenance Tool calibrates only the LSM scan head.
The System Maintainance Tool is for Service only. If a usewr tries to open the tool, the system
warns that "For ELYRA 7 the SMT is or Servie use only" (see Fig.x)
The following sections therefore descrivbe selected tool for Service.
1.1
Calibration Objective
Observe the following notes:
−
The calibration objective is not suitable for multiphoton excitation; there is a risk of bubble formation in
the substrate.
−
Using the HAL, the IR protection filter must be used, and the intensity of the HAL should only briefly be
set to more than 70 %.
−
With strong heat there is a risk of deformation of the substrate in the calibration objective.
−
The lateral screws need to be tightened carefully without applying too much force.
−
The calibration objective needs to be entered in the system database as "APO Calibration LSM" with
SAP no. 420639-9000-700. It needs to be entered at ZEN \ Maintain \ Objectives and at the TFT display.
−
Calibration objectives with other samples than shown in Fig. 2 shall not be used. The cap with the
calibration sample can be obtained separately under SAP-No. 420639-9900-700.
Fig. 1
System Maintainance Tool warning