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ZEISS
Hardware Aspects
ELYRA 7
16
000000-2262-999
03/2019 V_02
2.4
Microscope Equipment of ELYRA Systems
The ELYRA 7 systems are equipped with the inverted Axio Observer 7 SR microscope with a modified rear
port for the attachment of ELYRA illumination modules.
Only the differences from the delivered operating manual "Axio Observer" will be explained here.
(1) Stand
−
The motorized objective nosepiece 5
×
H DIC is firmly fixed to the stand, where no operating
elements can be found for the nosepiece. Operation will be performed via ZEN software control.
The "Restriction of the nosepiece height to protect the objectives during motorized objective
change" is inactivated. The nosepiece will be moved down automatically before each motorized
objective change.
−
The reflector mount is motorized and provided with the Axio Observer 7 SR reflector turret. The
reflector turret has six positions: One transmitting light position (position 1), which is empty, one or
more further positions (position 2-6) for push & click fluorescence filter sets or analyzer/polarizer
cubes (reflector module) to be used for optical observation. For ELYRA four of those positions
(positions 3-6) are adjusted and used in Lattice SIM and SMLM illumination regimes. For SMLM it is
also possible to use a push & click filter set. If you want to use more conventional fluorescence filter
sets, you need to employ a further reflector turret. When changing the reflector turret position you
must make sure that the turret will click into position, since otherwise the image area will be cut.
The reflector turrets are equipped with auto component recognition (ACR), so the actual filter sets
will be updated and displayed in the ZEN software.
−
The stand has a motorized focusing drive (fine & coarse). Sensitivity of the focusing drive is adjusted
to the delivered objectives by the manufacturer but can be modified using the controls on the TFT
display of the stand. The stand features an integrated power supply for the internal motors and
stand electronics. External power supply units are used for the mercury vapor short arc lamp or the
fiber coupled lamp.
−
The analyzer slider for conventional DIC methods will be operated from the right side and is located
just below the nosepiece.
−
When the rod is pushed in, the analyzer is located in the beam path. In the laser widefield modes
(Lattice SIM, SIM Apotome, SMLM, TIRF), the analyzer must not be located in the beam path, and
the analyzer rod must be pulled out.
−
When using SMLM, SIM Apotome and Lattice SIM, do not use a DIC slider.
(2) Specimen stages and fine focus drives
−
XY-Piezo Scanning stage for motorized positioning in X and Y.
−
Z-Piezo stage insert for motorized positioning in Z. The z-Piezo stage insert is obligatory for SR-SIM
illumination. It is highly recommended for SMLM illumination when employing the holding focus. It
is not compatible with Temp inserts and CO
2
-incubators.