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ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
205
5.3.18
Processing – SIM Tools
Licensing
Instrument
standard
optional
LSM
ELYRA
Lightsheet Z.1
Tool accepts data recorded in SIM mode on ELYRA. In data sets containing other
types of image data as well ("mixed mode" files), this tool selectively processes the
SIM data. "Experimental PSF" (requires Z-stacks of point-like objects) and "Fourier
Transform" accepts input other than SIM data as well. See full description of tool set
for details.
The
Processing
SIM tools
works exclusively for data
acquired in the
SIM Apotome
and
Stripe
&
Lattice
SIM imaging
modes with the exception of
Stripe
SIM
tool that only works for Stripe SIM. It subdivides
in 4 subcategories (Fig. 348):
−
Fourier transform
: Calculates the spatial
frequencies and displays them in an image. No
phase information will be available.
−
Modulation contrast
: Determines the modulation contrast from an image with beads to serve as
a diagnostic tool.
−
Stripe SIM
: calculates the high resolution image from the set of structured images acquoired with
linear grid pattern.
−
TIRF filtered
: Will apply the TIRF sectioning to the SIM image if the TIRF was added as the last
frame.
Fig. 339
Processing – SIM tools