![background image](http://html1.mh-extra.com/html/zeiss/elyra-7/elyra-7_operating-manual_3332235179.webp)
ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
000000-2262-999
175
5.3.9.1
Single Wavelength Dyes – Offline Calibration
•
Subtract background/ autofluorescence image from raw images to obtain better raw data to start with
•
Perform equation or titration calibration (compare F with a calibration curve
→
titration calibration or
put F values in calibration formula).
Fig. 279
Ion concentration panel