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ELYRA 7
Center Screen Area / Image Containers - Display and Image Analysis
ZEISS
03/2019 V_02
000000-2262-999
329
6.10
Colocalization View
The
Colocalization
function permits interactive
analysis of two channels of an image by computing
a scatter diagram (co-localization).
The settings of the
Dimensions
,
Display
,
Player
and
Graphics
view options control blocks apply.
The additional view-specific
Colocalization View
Option
control block is shown in Fig. 545.
Any changes done with this View Option control
block are effective immediately.
The Image Display in the Colocalization View shows 3 panels: the scatter diagram, the data table and the
pseudo-colored image display (see Fig. 546 and Fig. 547).
Colocalization is defined by the presence of two or more different molecules at the same location
in a specimen. However, in the context of digital imaging, the term colocalization refers to colors
emitted by fluorescent molecules detected by the same pixel in the image. It is important to be
aware of the fact that colocalization cannot be analyzed for fluorophores with similar emission
spectra. Accurate colocalization analysis is only possible if the fluorescence emission spectra are
well separated between fluorophores and the correct filter sets (or spectral detection bands) are
used for data acquisition. If spectral bleed-through artifacts are present because of spectral overlap
between the fluorophore emission spectra, or due to the use of incorrect filter sets, colocalization
measurements will be meaningless. To avoid this, the fluorophores must be carefully chosen and
matched to the excitation laser lines to obtain the maximum excitation efficiency while still
maintaining a useful degree of separation between emission wavelengths. The choice of
fluorophores is crucial for colocalization analysis.
Fig. 545
Colocalization View Option control
block